TY - GEN
T1 - Micro-CT sections and histological sections of mouse skull defects implanted with cartilage grown in a rotating bioreactor
AU - Duke, P. J.
AU - Montufar-Solis, D.
AU - Nguyen, H. C.
AU - Cody, D. D.
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2008
Y1 - 2008
N2 - Using cartilage to replace/repair bone is advantageous as no scaffolding is required to form the implant which disappears as bone is formed during the endochondral process. Previously, we demonstrated that cartilage spheroids, grown in a rotating bioreactor, (Synthecon, Inc.) and implanted into a 2 mm skull defect, contributed to healing of the defect. In this report, skulls with or without implants were subjected in microCT scan, and sections from these scans were compared to histological sections of the defect region of demineralized skulls from the same experiment. The area of the defect staining for bone in histological sections of demineralized skulls was the same region shown as mineralized in CT sections. Defects without implants were shown in serial CT sections and histological sections, to be incompletely healed. This study demonstrates that microCT scans are an important corollary to histological studies evaluating the use of implants in healing of bony defects. Supported in part by NIH/NIDCR Training Grant T35 DEO7252 and by Cancer Center Support Grant (CA-16672).
AB - Using cartilage to replace/repair bone is advantageous as no scaffolding is required to form the implant which disappears as bone is formed during the endochondral process. Previously, we demonstrated that cartilage spheroids, grown in a rotating bioreactor, (Synthecon, Inc.) and implanted into a 2 mm skull defect, contributed to healing of the defect. In this report, skulls with or without implants were subjected in microCT scan, and sections from these scans were compared to histological sections of the defect region of demineralized skulls from the same experiment. The area of the defect staining for bone in histological sections of demineralized skulls was the same region shown as mineralized in CT sections. Defects without implants were shown in serial CT sections and histological sections, to be incompletely healed. This study demonstrates that microCT scans are an important corollary to histological studies evaluating the use of implants in healing of bony defects. Supported in part by NIH/NIDCR Training Grant T35 DEO7252 and by Cancer Center Support Grant (CA-16672).
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M3 - Conference contribution
AN - SCOPUS:66949176558
SN - 9789292212278
T3 - European Space Agency, (Special Publication) ESA SP
BT - Proceedings of the Symposium Life in Space for Life on Earth
T2 - Life in Space for Life on Earth
Y2 - 22 June 2008 through 27 July 2008
ER -