TY - JOUR
T1 - Micrornas from liquid biopsy derived extracellular vesicles
T2 - Recent advances in detection and characterization methods
AU - Drula, Rares
AU - Ott, Leonie Florence
AU - Berindan-Neagoe, Ioana
AU - Pantel, Klaus
AU - Calin, George A.
N1 - Funding Information:
Klaus Pantel received funding from the European Research Council (ERC) Advanced Investigator Grants DISSECT and INJURMET, the European IMI research project CANCER-ID (115749-CANCER-ID), the Deutsche Krebshilfe (Nr. 70112504), and the Deutsche Forschungsgemeinschaft (DFG) SPP2084 µBone. This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 824036. Rares Drula has received an internal grant with title “The role of circular RNAs in lung cancer”—no. 2461/22 PCD founded by the “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania. Leonie Ott has received funding from the Deutsche Krebshilfe, Translationale Onkologie, grant 70112504. Acknowledgments: Calin is the Felix L. Haas Endowed Professor in Basic Science. Work in Calin’s laboratory is supported by National Institutes of Health (NIH/NCATS) grant UH3TR00943-01 through the NIH Common Fund, Office of Strategic Coordination (OSC), the NCI grants 1R01 CA182905-01 and 1R01CA222007-01A1, an NIGMS 1R01GM122775-01 grant, a U54 grant #CA096297/CA096300—UPR/MDACC Partnership for Excellence in Cancer Research 2016 Pilot Project, a Team DOD (CA160445P1) grant, a Chronic Lymphocytic Leukemia Moonshot Flagship project, a CLL Global research Foundation 2019 grant, a CLL Global research Foundation 2020 grant, and the Estate of C. G. Johnson, Jr.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/8
Y1 - 2020/8
N2 - Liquid biopsies have become a convenient tool in cancer diagnostics, real-time disease monitoring, and evaluation of residual disease. Yet, the information still encrypted in the variety of tumor-derived molecules identified in biofluids has proven difficult to decipher due to the technological limitations imposed by their biological nature. Such is the case of extracellular vesicle (EV) encapsulated ncRNAs, which have gained traction in recent years as biomarkers. Due to their resilience towards degrading factors they may act as suitable disease indicators. This review addresses the less described issues in this context. We present an overview of less investigated biofluids that can be used for EV isolation in addition to different isolation approaches to overcome the technical challenges these specimens harbor. Furthermore, we summarize the latest technological advances providing improvement to ncRNA detection and analysis. Thereby, this review summarizes the current state-of-the-art methodologies regarding EV and EV derived miRNA analysis and how they compare to current approaches.
AB - Liquid biopsies have become a convenient tool in cancer diagnostics, real-time disease monitoring, and evaluation of residual disease. Yet, the information still encrypted in the variety of tumor-derived molecules identified in biofluids has proven difficult to decipher due to the technological limitations imposed by their biological nature. Such is the case of extracellular vesicle (EV) encapsulated ncRNAs, which have gained traction in recent years as biomarkers. Due to their resilience towards degrading factors they may act as suitable disease indicators. This review addresses the less described issues in this context. We present an overview of less investigated biofluids that can be used for EV isolation in addition to different isolation approaches to overcome the technical challenges these specimens harbor. Furthermore, we summarize the latest technological advances providing improvement to ncRNA detection and analysis. Thereby, this review summarizes the current state-of-the-art methodologies regarding EV and EV derived miRNA analysis and how they compare to current approaches.
KW - Exosome
KW - Extracellular vesicle
KW - Liquid biopsy
KW - MiRNA biomarker
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U2 - 10.3390/cancers12082009
DO - 10.3390/cancers12082009
M3 - Review article
C2 - 32707943
AN - SCOPUS:85088295540
SN - 2072-6694
VL - 12
SP - 1
EP - 24
JO - Cancers
JF - Cancers
IS - 8
M1 - 2009
ER -