@article{a61da31b1b3e4eb4ac6c93995f61a385,
title = "Microtubule destabilization is a critical checkpoint of chemotaxis and transendothelial migration in melanoma cells but not in T cells",
abstract = "Microtubules (MTs) control cell shape and intracellular cargo transport. The role of MT turnover in the migration of slow-moving cells through endothelial barriers remains unclear. To irreversibly interfere with MT disassembly, we have used the MT-stabilizing agent zampanolide (ZMP) in Β16F10 melanoma as amodel of slow-moving cells. ZMP-treated B16 cells failed to follow chemotactic gradients across rigid confinements and could not generate stable sub-endothelial pseudopodia under endothelial monolayers. In vivo, ZMP-treated Β16 cells failed to extravasate though lung capillaries. In contrast to melanoma cells, the chemotaxis and transendothelial migration of ZMP-treated Tcells were largely conserved. This is afirst demonstration that MT disassembly is akey checkpoint in the directional migration of cancer cells but not of lymphocytes.",
keywords = "Cytoskeleton, cancer, metastasis, motility, taxol",
author = "Francesco Roncato and Ofer Regev and Yadav, {Sandeep Kumar} and Ronen Alon",
note = "Funding Information: We thank Prof. Karl-Heinz Altmann (Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland) for the synthesis of zampanolide and Dr. Jos{\'e} Fernando D{\'i}az (Centro de Investigaciones Biol{\'o}gicas, CSIC, Madrid, Spain) for the characterization of the compound. We thank Dr. Sara W. Feigelson (WIS) for helpful suggestions and discussions. We also thank Dr. Yoseph Addadi (Life Sciences Core Facilities, WIS) for help with LSFM and analysis. R.A. is the Incumbent of the Linda Jacobs Chair in Immune and Stem Cell Research. His research is supported by the Israel Science Foundation (grant no. 791/17), the Minerva Stiftung, GIF (grant number I-1470-412.13/2018), as well as grants from the Moross Integrated Cancer Center, Yeda-Sela Center for Basic Research, Helen and Martin Kimmel Institute for Stem Cell Research, the Meyer Henri Cancer Endowment, and from William and Marika Glied and Carol A. Milett. Funding Information: This work was supported by the German-Israeli Foundation for Scientific Research and Development [I-1470-412.13/2018]; Israel Science Foundation [791/17]; Minerva Foundation; Helen and Martin Kimmel Institute for Stem Cell Research; Yeda-Sela Center for Basic Research; The Meyer Henri Cancer Endowment; Moross Integrated Cancer Center. We thank Prof. Karl-Heinz Altmann (Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland) for the synthesis of zampanolide and Dr. Jos? Fernando D?az (Centro de Investigaciones Biol?gicas, CSIC, Madrid, Spain) for the characterization of the compound. We thank Dr. Sara W. Feigelson (WIS) for helpful suggestions and discussions. We also thank Dr. Yoseph Addadi (Life Sciences Core Facilities, WIS) for help with LSFM and analysis. R.A. is the Incumbent of the Linda Jacobs Chair in Immune and Stem Cell Research. His research is supported by the Israel Science Foundation (grant no. 791/17), the Minerva Stiftung, GIF (grant number I-1470-412.13/2018), as well as grants from the Moross Integrated Cancer Center, Yeda-Sela Center for Basic Research, Helen and Martin Kimmel Institute for Stem Cell Research, the Meyer Henri Cancer Endowment, and from William and Marika Glied and Carol A. Milett. Publisher Copyright: {\textcopyright} 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",
year = "2021",
doi = "10.1080/19336918.2021.1934958",
language = "English (US)",
volume = "15",
pages = "166--179",
journal = "Cell Adhesion and Migration",
issn = "1933-6918",
publisher = "Landes Bioscience",
number = "1",
}