TY - JOUR
T1 - Modification of erythrocyte membrane proteins, enzymes and transport mechanisms in chronic alcoholics
T2 - An in vivo and in vitro study
AU - Maturu, Paramahamsa
AU - Vaddi, Damodara Reddy
AU - Pannuru, Padmavathi
AU - Nallanchakravarthula, Varadacharyulu
N1 - Funding Information:
Funding — This study was supported in part by the University Grants Commission (Grant No. F-3-11/97), New Delhi, India.
PY - 2013/11
Y1 - 2013/11
N2 - Aim: The aim of the study was to elucidate the molecular mechanisms underlying the alcohol perturbation leading to deleterious effects on erythrocyte membrane transport in chronic alcoholics. Methods: Membrane bound enzyme activities such as Na+, K+-ATPase, Ca2+,Mg2+-ATPase and acetylcholine esterase and membrane transport analysis by in vitro and erythrocyte membraneprofile analysis in controls and chronic alcoholic red cells were analyzed.Results: It was observed that decreased Na+, K+-ATPase enzyme activity and increasedactivities of Ca2+,Mg2+-ATPase and acetylcholine esterase in chronic alcoholics compared to controls. The in vitro studies of erythrocytes suggested that there is an increased uptake of glucose through chronic alcoholic red cells. However, glucose utilization by chronic alcoholic red cellswas decreased. An increased sensitivity of ouabain for its binding site on Na+, K+- ATPase in chronic alcoholic erythrocyte membrane was evident from this study. Though there appears to be anincreased Na+ influx in chronic alcoholic cells, the status of Na+ transport is not altered much. However, ouabain caused slightdisturbances in the transport of sodium, similar disturbances in the potassium transport resulting in much accumulation of potassium in red cells. Conclusions: It was concluded that chronic alcohol consumption modified certain membrane bound proteins, enzymes and transport mechanisms in chronic alcoholics.
AB - Aim: The aim of the study was to elucidate the molecular mechanisms underlying the alcohol perturbation leading to deleterious effects on erythrocyte membrane transport in chronic alcoholics. Methods: Membrane bound enzyme activities such as Na+, K+-ATPase, Ca2+,Mg2+-ATPase and acetylcholine esterase and membrane transport analysis by in vitro and erythrocyte membraneprofile analysis in controls and chronic alcoholic red cells were analyzed.Results: It was observed that decreased Na+, K+-ATPase enzyme activity and increasedactivities of Ca2+,Mg2+-ATPase and acetylcholine esterase in chronic alcoholics compared to controls. The in vitro studies of erythrocytes suggested that there is an increased uptake of glucose through chronic alcoholic red cells. However, glucose utilization by chronic alcoholic red cellswas decreased. An increased sensitivity of ouabain for its binding site on Na+, K+- ATPase in chronic alcoholic erythrocyte membrane was evident from this study. Though there appears to be anincreased Na+ influx in chronic alcoholic cells, the status of Na+ transport is not altered much. However, ouabain caused slightdisturbances in the transport of sodium, similar disturbances in the potassium transport resulting in much accumulation of potassium in red cells. Conclusions: It was concluded that chronic alcohol consumption modified certain membrane bound proteins, enzymes and transport mechanisms in chronic alcoholics.
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U2 - 10.1093/alcalc/agt071
DO - 10.1093/alcalc/agt071
M3 - Article
C2 - 23966453
AN - SCOPUS:84886287135
SN - 0735-0414
VL - 48
SP - 679
EP - 686
JO - Alcohol and Alcoholism
JF - Alcohol and Alcoholism
IS - 6
M1 - agt071
ER -