Modified methodology to improve flow cytometric dna histograms from paraffin-embedded material

Donia D. Mclemore; Adel El Naggar; L. Clifton Stephens; John H. Jardine

doi:10.3109/10520299009105619

Modified methodology to improve flow cytometric dna histograms from paraffin-embedded material

Donia D. Mclemore, Adel El Naggar, L. Clifton Stephens, John H. Jardine

Pathology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Flow Cytometric (FCM) DNA content analysis of paraffin-embedded tissues has become a widely accepted procedure in assessing the biologic course in some tumors from archival material. Difficulty in interpreting histograms is frequently due to high levels of debris, and wide coefficients of variation (CV). These may lead to underestimating near-diploid abnormality. Although the clinical significance of low-degree aneuploidy has yet to be established, the procedure reported here improved our endeavor to detect DNA Indices (DI) of at least 1.1% Experience has shown that careful technique can result in overall improvement of DNA histograms by lowering levels of debris and % CV, dramatically so in some cases. Archival histograms can be generated that rival in quality fresh tissue results. Archival material is currently employed for diagnostic and research purposes.

Original language	English (US)
Pages (from-to)	279-291
Number of pages	13
Journal	Biotechnic and Histochemistry
Volume	65
Issue number	6
DOIs	https://doi.org/10.3109/10520299009105619
State	Published - 1990

ASJC Scopus subject areas

Histology
Medical Laboratory Technology

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title = "Modified methodology to improve flow cytometric dna histograms from paraffin-embedded material",

abstract = "Flow Cytometric (FCM) DNA content analysis of paraffin-embedded tissues has become a widely accepted procedure in assessing the biologic course in some tumors from archival material. Difficulty in interpreting histograms is frequently due to high levels of debris, and wide coefficients of variation (CV). These may lead to underestimating near-diploid abnormality. Although the clinical significance of low-degree aneuploidy has yet to be established, the procedure reported here improved our endeavor to detect DNA Indices (DI) of at least 1.1% Experience has shown that careful technique can result in overall improvement of DNA histograms by lowering levels of debris and % CV, dramatically so in some cases. Archival histograms can be generated that rival in quality fresh tissue results. Archival material is currently employed for diagnostic and research purposes.",

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AU - Mclemore, Donia D.

AU - Naggar, Adel El

AU - Stephens, L. Clifton

AU - Jardine, John H.

PY - 1990

Y1 - 1990

N2 - Flow Cytometric (FCM) DNA content analysis of paraffin-embedded tissues has become a widely accepted procedure in assessing the biologic course in some tumors from archival material. Difficulty in interpreting histograms is frequently due to high levels of debris, and wide coefficients of variation (CV). These may lead to underestimating near-diploid abnormality. Although the clinical significance of low-degree aneuploidy has yet to be established, the procedure reported here improved our endeavor to detect DNA Indices (DI) of at least 1.1% Experience has shown that careful technique can result in overall improvement of DNA histograms by lowering levels of debris and % CV, dramatically so in some cases. Archival histograms can be generated that rival in quality fresh tissue results. Archival material is currently employed for diagnostic and research purposes.

AB - Flow Cytometric (FCM) DNA content analysis of paraffin-embedded tissues has become a widely accepted procedure in assessing the biologic course in some tumors from archival material. Difficulty in interpreting histograms is frequently due to high levels of debris, and wide coefficients of variation (CV). These may lead to underestimating near-diploid abnormality. Although the clinical significance of low-degree aneuploidy has yet to be established, the procedure reported here improved our endeavor to detect DNA Indices (DI) of at least 1.1% Experience has shown that careful technique can result in overall improvement of DNA histograms by lowering levels of debris and % CV, dramatically so in some cases. Archival histograms can be generated that rival in quality fresh tissue results. Archival material is currently employed for diagnostic and research purposes.

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Modified methodology to improve flow cytometric dna histograms from paraffin-embedded material

Abstract

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