TY - JOUR
T1 - Modulation of the urokinase receptor in human colon cell lines by N,N-dimethylformamide
AU - Boyd, Douglas
AU - Florent, Germaine
AU - Murano, Genesio
AU - Brattain, Michael
N1 - Funding Information:
This work was supported by a NIH Grant CA 34432.
PY - 1988/6/8
Y1 - 1988/6/8
N2 - The present study documents the effect of the planar, polar differentiation promoter N,N-dimethylformamide (DMF) on urokinase binding to colon carcinoma cells. Exposure of the colon carcinoma-cell lines to the agent resulted in enhanced specific binding of radioactive urokinase to all cells tested. Insulin binding to the cells was, however, unaffected by DMF. A DMF exposure period of 45 h was required to observe maximum urokinase binding to two representative cell lines FET and RKO. Optimal stimulation of both cell lines occurred with 0.8% DMF. Scatchard analysis revealed the dissociation constants to be unchanged by the agent with the increased binding of radioactive plasminogen activator reflecting an up-regulation of binding sites. In this regard, the cell line RKO upon exposure to DMF, displayed approx. 700000 receptors/cell, the highest value published, to date, for any cell line.
AB - The present study documents the effect of the planar, polar differentiation promoter N,N-dimethylformamide (DMF) on urokinase binding to colon carcinoma cells. Exposure of the colon carcinoma-cell lines to the agent resulted in enhanced specific binding of radioactive urokinase to all cells tested. Insulin binding to the cells was, however, unaffected by DMF. A DMF exposure period of 45 h was required to observe maximum urokinase binding to two representative cell lines FET and RKO. Optimal stimulation of both cell lines occurred with 0.8% DMF. Scatchard analysis revealed the dissociation constants to be unchanged by the agent with the increased binding of radioactive plasminogen activator reflecting an up-regulation of binding sites. In this regard, the cell line RKO upon exposure to DMF, displayed approx. 700000 receptors/cell, the highest value published, to date, for any cell line.
KW - N,N-Dimethylformamide
KW - Receptor modulation
KW - Urokinase receptor
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U2 - 10.1016/0167-4889(88)90227-3
DO - 10.1016/0167-4889(88)90227-3
M3 - Article
C2 - 2835992
AN - SCOPUS:0023879751
SN - 0167-4889
VL - 970
SP - 96
EP - 100
JO - BBA - Molecular Cell Research
JF - BBA - Molecular Cell Research
IS - 1
ER -