TY - JOUR
T1 - Molecular alterations in the tumor suppressor gene WWOX in oral leukoplakias
AU - Pimenta, Flávio Juliano
AU - Cordeiro, Gabriela Tavares
AU - Pimenta, Luiz Gustavo Garcia Santos
AU - Viana, Michelle Beatriz
AU - Lopes, Joyce
AU - Gomez, Marcus Vinícius
AU - Aldaz, C. Marcelo
AU - De Marco, Luiz
AU - Gomez, Ricardo Santiago
PY - 2008/8
Y1 - 2008/8
N2 - Oral leukoplakia is the most prevalent and potentially malignant disorder of the oral mucosa. Previous studies have demonstrated that molecular changes of the WWOX gene (WW-domain containing oxidoreductase), a candidate tumor suppressor gene located at 16q23.3-24.1 that spans FRA16D, the second most common fragile site, are present in several malignant neoplasias, including oral squamous cell carcinoma. In this report, the role of the WWOX gene was investigated in 23 cases of oral leukoplakias. Using nested RT-PCR and immunohistochemistry, altered mRNA transcription and/or reduced Wwox protein expression was observed in 35% of the lesions when compared with normal mucosa. The majority of lesions (4/6) with altered transcripts had a reduction in the expression of Wwox protein. Although normal WWOX expression was found in some lesions with dysplasia, all lesions with WWOX mRNA and/or protein expression showed histological evidence of dysplasia and none of the cases without dysplasia presented this alteration. These results show that the WWOX gene alteration is an early genetic alteration and may contribute to oral carcinogenesis.
AB - Oral leukoplakia is the most prevalent and potentially malignant disorder of the oral mucosa. Previous studies have demonstrated that molecular changes of the WWOX gene (WW-domain containing oxidoreductase), a candidate tumor suppressor gene located at 16q23.3-24.1 that spans FRA16D, the second most common fragile site, are present in several malignant neoplasias, including oral squamous cell carcinoma. In this report, the role of the WWOX gene was investigated in 23 cases of oral leukoplakias. Using nested RT-PCR and immunohistochemistry, altered mRNA transcription and/or reduced Wwox protein expression was observed in 35% of the lesions when compared with normal mucosa. The majority of lesions (4/6) with altered transcripts had a reduction in the expression of Wwox protein. Although normal WWOX expression was found in some lesions with dysplasia, all lesions with WWOX mRNA and/or protein expression showed histological evidence of dysplasia and none of the cases without dysplasia presented this alteration. These results show that the WWOX gene alteration is an early genetic alteration and may contribute to oral carcinogenesis.
KW - Oral carcinogenesis
KW - Oral leukoplakia
KW - Oral premalignant lesion
KW - Tumor suppressor gene
KW - WWOX gene
UR - http://www.scopus.com/inward/record.url?scp=47249089230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=47249089230&partnerID=8YFLogxK
U2 - 10.1016/j.oraloncology.2007.08.019
DO - 10.1016/j.oraloncology.2007.08.019
M3 - Article
C2 - 18061530
AN - SCOPUS:47249089230
SN - 1368-8375
VL - 44
SP - 753
EP - 758
JO - Oral Oncology
JF - Oral Oncology
IS - 8
ER -