Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex

Limin Gong; Bing Li; Stefanos Millas; Edward T.H. Yeh

doi:10.1016/S0014-5793(99)00367-1

Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex

Limin Gong, Bing Li, Stefanos Millas, Edward T.H. Yeh

Cardiology

Research output: Contribution to journal › Article › peer-review

134 Scopus citations

Abstract

Sentrin-1/SUMO-1 is a novel ubiquitin-like protein, which can covalently modify a limited number of cellular proteins. Here we report the identification of the sentrin-activating enzyme complex, which consists of two proteins AOS1 and UBA2. Human AOS1 is homologous to the N-terminal half of E1, whereas human UBA2 is homologous to the C-terminal half of E1. The human UBA2 gene is located on chromosome 19q12. Human UBA2 could form a β-mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8, in the presence of AOS1. Identification of human UBA2 and AOS1 should allow a more detailed analysis of the enzymology of the activation of ubiquitin-like proteins. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	185-189
Number of pages	5
Journal	FEBS Letters
Volume	448
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(99)00367-1
State	Published - Apr 2 1999

Keywords

AOS1
SUMO
Sentrin
UBA2
UBC9
Ubiquitin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)00367-1

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title = "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex",

abstract = "Sentrin-1/SUMO-1 is a novel ubiquitin-like protein, which can covalently modify a limited number of cellular proteins. Here we report the identification of the sentrin-activating enzyme complex, which consists of two proteins AOS1 and UBA2. Human AOS1 is homologous to the N-terminal half of E1, whereas human UBA2 is homologous to the C-terminal half of E1. The human UBA2 gene is located on chromosome 19q12. Human UBA2 could form a β-mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8, in the presence of AOS1. Identification of human UBA2 and AOS1 should allow a more detailed analysis of the enzymology of the activation of ubiquitin-like proteins. Copyright (C) 1999 Federation of European Biochemical Societies.",

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author = "Limin Gong and Bing Li and Stefanos Millas and Yeh, {Edward T.H.}",

note = "Funding Information: We would like to thank L.S. Caskey for technical help. This work was supported in part by National Institute of Health Grant HL-45851 and an Established Investigator Award from the American Heart Association (to E.T.H.Y.). ",

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AU - Millas, Stefanos

AU - Yeh, Edward T.H.

N1 - Funding Information: We would like to thank L.S. Caskey for technical help. This work was supported in part by National Institute of Health Grant HL-45851 and an Established Investigator Award from the American Heart Association (to E.T.H.Y.).

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N2 - Sentrin-1/SUMO-1 is a novel ubiquitin-like protein, which can covalently modify a limited number of cellular proteins. Here we report the identification of the sentrin-activating enzyme complex, which consists of two proteins AOS1 and UBA2. Human AOS1 is homologous to the N-terminal half of E1, whereas human UBA2 is homologous to the C-terminal half of E1. The human UBA2 gene is located on chromosome 19q12. Human UBA2 could form a β-mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8, in the presence of AOS1. Identification of human UBA2 and AOS1 should allow a more detailed analysis of the enzymology of the activation of ubiquitin-like proteins. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Sentrin-1/SUMO-1 is a novel ubiquitin-like protein, which can covalently modify a limited number of cellular proteins. Here we report the identification of the sentrin-activating enzyme complex, which consists of two proteins AOS1 and UBA2. Human AOS1 is homologous to the N-terminal half of E1, whereas human UBA2 is homologous to the C-terminal half of E1. The human UBA2 gene is located on chromosome 19q12. Human UBA2 could form a β-mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8, in the presence of AOS1. Identification of human UBA2 and AOS1 should allow a more detailed analysis of the enzymology of the activation of ubiquitin-like proteins. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - AOS1

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KW - Sentrin

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KW - Ubiquitin

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Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex

Abstract

Keywords

ASJC Scopus subject areas

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