Molecular detection of t(11;14)(q13;q32) in mantle cell lymphoma

C. Cameron Yin; Rajyalakshmi Luthra

doi:10.1007/978-1-62703-357-2_14

Molecular detection of t(11;14)(q13;q32) in mantle cell lymphoma

C. Cameron Yin, Rajyalakshmi Luthra

Hematopathology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

Mantle cell lymphoma (MCL) is characterized by the presence of t(11;14)(q13;q32) which juxtaposes CCND-1 gene (also known as BCL-1, PRAD-1) at 11q13 with an enhancer of the IGH gene at 14q32. The resultant overexpression of cyclin D1 plays an essential role in the pathogenesis of MCL. The breakpoints on chromosome 14 occur 5′ to one of six J_H segments, whereas only 30-50% of the breakpoints on chromosome 11 are localized within a 1 kb region called the major translocation cluster (MTC) which can be easily assessed by polymerase chain reaction (PCR). The remainder of the breakpoints are widely scattered over approximately 120 kb, making PCR analysis infeasible. We describe a TaqMan-based real-time PCR assay to detect and quantify IGH/BCL1 fusion products in newly diagnosed MCL, and to monitor minimal residual disease during treatment or early relapse in MTC-positive cases.

Original language	English (US)
Title of host publication	Hematological Malignancies
Publisher	Humana Press Inc.
Pages	211-216
Number of pages	6
ISBN (Print)	9781627033565
DOIs	https://doi.org/10.1007/978-1-62703-357-2_14
State	Published - 2013

Publication series

Name	Methods in Molecular Biology
Volume	999
ISSN (Print)	1064-3745

Keywords

Mantle cell lymphoma
PCR
t(11;14)(q13;q32)

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-62703-357-2_14

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title = "Molecular detection of t(11;14)(q13;q32) in mantle cell lymphoma",

abstract = "Mantle cell lymphoma (MCL) is characterized by the presence of t(11;14)(q13;q32) which juxtaposes CCND-1 gene (also known as BCL-1, PRAD-1) at 11q13 with an enhancer of the IGH gene at 14q32. The resultant overexpression of cyclin D1 plays an essential role in the pathogenesis of MCL. The breakpoints on chromosome 14 occur 5′ to one of six JH segments, whereas only 30-50% of the breakpoints on chromosome 11 are localized within a 1 kb region called the major translocation cluster (MTC) which can be easily assessed by polymerase chain reaction (PCR). The remainder of the breakpoints are widely scattered over approximately 120 kb, making PCR analysis infeasible. We describe a TaqMan-based real-time PCR assay to detect and quantify IGH/BCL1 fusion products in newly diagnosed MCL, and to monitor minimal residual disease during treatment or early relapse in MTC-positive cases.",

keywords = "Mantle cell lymphoma, PCR, t(11;14)(q13;q32)",

author = "Yin, {C. Cameron} and Rajyalakshmi Luthra",

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TY - CHAP

T1 - Molecular detection of t(11;14)(q13;q32) in mantle cell lymphoma

AU - Yin, C. Cameron

AU - Luthra, Rajyalakshmi

PY - 2013

Y1 - 2013

N2 - Mantle cell lymphoma (MCL) is characterized by the presence of t(11;14)(q13;q32) which juxtaposes CCND-1 gene (also known as BCL-1, PRAD-1) at 11q13 with an enhancer of the IGH gene at 14q32. The resultant overexpression of cyclin D1 plays an essential role in the pathogenesis of MCL. The breakpoints on chromosome 14 occur 5′ to one of six JH segments, whereas only 30-50% of the breakpoints on chromosome 11 are localized within a 1 kb region called the major translocation cluster (MTC) which can be easily assessed by polymerase chain reaction (PCR). The remainder of the breakpoints are widely scattered over approximately 120 kb, making PCR analysis infeasible. We describe a TaqMan-based real-time PCR assay to detect and quantify IGH/BCL1 fusion products in newly diagnosed MCL, and to monitor minimal residual disease during treatment or early relapse in MTC-positive cases.

AB - Mantle cell lymphoma (MCL) is characterized by the presence of t(11;14)(q13;q32) which juxtaposes CCND-1 gene (also known as BCL-1, PRAD-1) at 11q13 with an enhancer of the IGH gene at 14q32. The resultant overexpression of cyclin D1 plays an essential role in the pathogenesis of MCL. The breakpoints on chromosome 14 occur 5′ to one of six JH segments, whereas only 30-50% of the breakpoints on chromosome 11 are localized within a 1 kb region called the major translocation cluster (MTC) which can be easily assessed by polymerase chain reaction (PCR). The remainder of the breakpoints are widely scattered over approximately 120 kb, making PCR analysis infeasible. We describe a TaqMan-based real-time PCR assay to detect and quantify IGH/BCL1 fusion products in newly diagnosed MCL, and to monitor minimal residual disease during treatment or early relapse in MTC-positive cases.

KW - Mantle cell lymphoma

KW - PCR

KW - t(11;14)(q13;q32)

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AN - SCOPUS:84883225877

SN - 9781627033565

T3 - Methods in Molecular Biology

SP - 211

EP - 216

BT - Hematological Malignancies

PB - Humana Press Inc.

ER -

Molecular detection of t(11;14)(q13;q32) in mantle cell lymphoma

Abstract

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Keywords

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