TY - JOUR
T1 - Molecular identification of the human tumor necrosis factor receptor on interleukin-2-stimulated peripheral blood lymphocytes
AU - Crump, William L.
AU - Owen-Schaub, Laurie B.
AU - Grimm, Elizabeth Ann
N1 - Funding Information:
’ This work was supported in part by U.S. Public Health Service Grant CA45225 (E.A.G.). 2 To whom correspondence and reprint requests should be addressed. 3 Abbreviations used: TNF, tumor necrosis factor; BS3,b is(sulfosuccinimidy1) suberate; SASD, sulfosuccinimidyl 2-(p-azido-salicylamido) ethyl 1J -dithiopropionate.
PY - 1990/11
Y1 - 1990/11
N2 - The human tumor necrosis factor (TNF) receptor on interleukin (IL)-2-stimulated lymphocytes was characterized by binding and crosslinking techniques. The TNF receptor on IL-2-activated lymphocytes has an affinity of approximately 50 pm. Conventional crosslinking studies with the DSS analog bis(sulfosuccinimidyl) suberate demonstrated a ligand-receptor complex molecular weight of 106-108 kDa. Lectin precipitation experiments indicated that the receptor is a glycoprotein with an affinity for lectin isolated from Ricinus communis. Affinity crosslinking studies with the iodinateable, cleavable crosslinker sulfosuccinimidyl 2-(p-azido-salicylamido) ethyl 1,3′-dithiopropionate demonstrated that the TNF receptor, by itself, in the absence of bound ligand, has a molecular weight of approximately 90 kDa. Furthermore, these results indicate that the crosslinked TNF:TNF-receptor complexes observed at 104-108 kDa are composed of receptor and monomeric TNF.
AB - The human tumor necrosis factor (TNF) receptor on interleukin (IL)-2-stimulated lymphocytes was characterized by binding and crosslinking techniques. The TNF receptor on IL-2-activated lymphocytes has an affinity of approximately 50 pm. Conventional crosslinking studies with the DSS analog bis(sulfosuccinimidyl) suberate demonstrated a ligand-receptor complex molecular weight of 106-108 kDa. Lectin precipitation experiments indicated that the receptor is a glycoprotein with an affinity for lectin isolated from Ricinus communis. Affinity crosslinking studies with the iodinateable, cleavable crosslinker sulfosuccinimidyl 2-(p-azido-salicylamido) ethyl 1,3′-dithiopropionate demonstrated that the TNF receptor, by itself, in the absence of bound ligand, has a molecular weight of approximately 90 kDa. Furthermore, these results indicate that the crosslinked TNF:TNF-receptor complexes observed at 104-108 kDa are composed of receptor and monomeric TNF.
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U2 - 10.1016/0008-8749(90)90242-J
DO - 10.1016/0008-8749(90)90242-J
M3 - Article
C2 - 2171784
AN - SCOPUS:0025222164
SN - 0008-8749
VL - 131
SP - 150
EP - 158
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -