TY - GEN
T1 - Molecular specific photoacoustic imaging with plasmonic nanosensors
AU - Mallidi, Srivalleesha
AU - Yantsen, Evgeniya
AU - Larson, Timothy
AU - Sokolov, Konstantin
AU - Emelianov, Stanislav
PY - 2008
Y1 - 2008
N2 - Gold nanoparticles functionalized with anti-EGFR antibodies undergo molecular specific aggregation on the cellular membrane and later within the cell that leads to a red shift in the plasmon resonance frequency of the gold nanoparticles. Capitalizing on this effect, we previously demonstrated on tissue phantoms that highly sensitive and selective detection of cancer cells can be achieved using the combination of photoacoustic imaging and molecular specific gold nanoparticles. To further evaluate the efficacy of molecular specific photoacoustic imaging technique in detecting deeply situated tumors, small animal experiments were performed. In this study, two gelatin solutions mixed with cells labeled with gold nanoparticles and cells mixed with polyethylene glycol-thiol (mPEG-SH) coated gold nanoparticles were injected in a mouse abdomen ex-vivo. The photoacoustic and ultrasound images from the same crosssection of the region before and after the injections were obtained using a 25 MHz single element ultrasound transducer interfaced with pulsed laser system. The results of our study suggest that the molecular specific photoacoustic imaging with plasmonic nanosensors could be used to detect deeply embedded tumors.
AB - Gold nanoparticles functionalized with anti-EGFR antibodies undergo molecular specific aggregation on the cellular membrane and later within the cell that leads to a red shift in the plasmon resonance frequency of the gold nanoparticles. Capitalizing on this effect, we previously demonstrated on tissue phantoms that highly sensitive and selective detection of cancer cells can be achieved using the combination of photoacoustic imaging and molecular specific gold nanoparticles. To further evaluate the efficacy of molecular specific photoacoustic imaging technique in detecting deeply situated tumors, small animal experiments were performed. In this study, two gelatin solutions mixed with cells labeled with gold nanoparticles and cells mixed with polyethylene glycol-thiol (mPEG-SH) coated gold nanoparticles were injected in a mouse abdomen ex-vivo. The photoacoustic and ultrasound images from the same crosssection of the region before and after the injections were obtained using a 25 MHz single element ultrasound transducer interfaced with pulsed laser system. The results of our study suggest that the molecular specific photoacoustic imaging with plasmonic nanosensors could be used to detect deeply embedded tumors.
KW - Gold nanoparticles
KW - Molecular imaging
KW - Optoacoustic imaging
KW - Photoacoustic imaging
KW - Plasmonic nanosensors
KW - Thermoacoustic imaging
UR - http://www.scopus.com/inward/record.url?scp=42149144125&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42149144125&partnerID=8YFLogxK
U2 - 10.1117/12.763903
DO - 10.1117/12.763903
M3 - Conference contribution
AN - SCOPUS:42149144125
SN - 9780819470317
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Photons Plus Ultrasound
T2 - 9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008
Y2 - 20 January 2008 through 23 January 2008
ER -