TY - JOUR
T1 - Molecular Techniques for Genus and Species Determination of Fungi from Fresh and Paraffin-Embedded Formalin-Fixed Tissue in the Revised EORTC/MSGERC Definitions of Invasive Fungal Infection
AU - Lockhart, Shawn R.
AU - Bialek, Ralf
AU - Kibbler, Christopher C.
AU - Cuenca-Estrella, Manuel
AU - Jensen, Henrik E.
AU - Kontoyiannis, Dimitrios P.
N1 - Publisher Copyright:
© 2021 The Author(s). Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.
PY - 2021/3/15
Y1 - 2021/3/15
N2 - The EORTC/MSGERC have revised the definitions for proven, probable, and possible fungal diseases. The tissue diagnosis subcommittee was tasked with determining how and when species can be determined from tissue in the absence of culture. The subcommittee reached a consensus decision that polymerase chain reaction (PCR) from tissue, but not immunohistochemistry or in situ hybridization, can be used for genus or species determination under the new EORTC/MSGERC guidelines, but only when fungal elements are identified by histology. Fungal elements seen in tissue samples by histopathology and identified by PCR followed by sequencing should fulfill the definition of a proven fungal infection, identified to genus/species, even in the absence of culture. This summary discusses the issues that were deliberated by the subcommittee to reach the consensus decision and outlines the criteria a laboratory should follow in order to produce data that meet the EORTC/MSGERC definitions.
AB - The EORTC/MSGERC have revised the definitions for proven, probable, and possible fungal diseases. The tissue diagnosis subcommittee was tasked with determining how and when species can be determined from tissue in the absence of culture. The subcommittee reached a consensus decision that polymerase chain reaction (PCR) from tissue, but not immunohistochemistry or in situ hybridization, can be used for genus or species determination under the new EORTC/MSGERC guidelines, but only when fungal elements are identified by histology. Fungal elements seen in tissue samples by histopathology and identified by PCR followed by sequencing should fulfill the definition of a proven fungal infection, identified to genus/species, even in the absence of culture. This summary discusses the issues that were deliberated by the subcommittee to reach the consensus decision and outlines the criteria a laboratory should follow in order to produce data that meet the EORTC/MSGERC definitions.
KW - EORTC/MSG
KW - FFPE
KW - formalin-fixed paraffin-embedded tissue
KW - immunohistochemistry
KW - tissue diagnosis
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U2 - 10.1093/cid/ciaa1836
DO - 10.1093/cid/ciaa1836
M3 - Article
C2 - 33709128
AN - SCOPUS:85102823494
SN - 1058-4838
VL - 72
SP - S109-S113
JO - Clinical Infectious Diseases
JF - Clinical Infectious Diseases
ER -