TY - JOUR
T1 - Monte Carlo model to describe depth selective fluorescence spectra of epithelial tissue
T2 - Applications for diagnosis of oral precancer
AU - Pavlova, Ina
AU - Weber, Crystal Redden
AU - Schwarz, Richard A.
AU - Williams, Michelle
AU - El-Naggar, Adel
AU - Gillenwater, Ann
AU - Richards-Kortum, Rebecca
N1 - Funding Information:
This work was supported by NIH grant R01 CA095604.
PY - 2008
Y1 - 2008
N2 - We present a Monte Carlo model to predict fluorescence spectra of the oral mucosa obtained with a depth-selective fiber optic probe as a function of tissue optical properties. A model sensitivity analysis determines how variations in optical parameters associated with neoplastic development influence the intensity and shape of spectra, and elucidates the biological basis for differences in spectra from normal and premalignant oral sites. Predictions indicate that spectra of oral mucosa collected with a depth-selective probe are affected by variations in epithelial optical properties, and to a lesser extent, by changes in superficial stromal parameters, but not by changes in the optical properties of deeper stroma. The depth selective probe offers enhanced detection of epithelial fluorescence, with 90% of the detected signal originating from the epithelium and superficial stroma. Predicted depth-selective spectra are in good agreement with measured average spectra from normal and dysplastic oral sites. Changes in parameters associated with dysplastic progression lead to a decreased fluorescence intensity and a shift of the spectra to longer emission wavelengths. Decreased fluorescence is due to a drop in detected stromal photons, whereas the shift of spectral shape is attributed to an increased fraction of detected photons arising in the epithelium.
AB - We present a Monte Carlo model to predict fluorescence spectra of the oral mucosa obtained with a depth-selective fiber optic probe as a function of tissue optical properties. A model sensitivity analysis determines how variations in optical parameters associated with neoplastic development influence the intensity and shape of spectra, and elucidates the biological basis for differences in spectra from normal and premalignant oral sites. Predictions indicate that spectra of oral mucosa collected with a depth-selective probe are affected by variations in epithelial optical properties, and to a lesser extent, by changes in superficial stromal parameters, but not by changes in the optical properties of deeper stroma. The depth selective probe offers enhanced detection of epithelial fluorescence, with 90% of the detected signal originating from the epithelium and superficial stroma. Predicted depth-selective spectra are in good agreement with measured average spectra from normal and dysplastic oral sites. Changes in parameters associated with dysplastic progression lead to a decreased fluorescence intensity and a shift of the spectra to longer emission wavelengths. Decreased fluorescence is due to a drop in detected stromal photons, whereas the shift of spectral shape is attributed to an increased fraction of detected photons arising in the epithelium.
KW - Light propagation
KW - Monte Carlo modeling
KW - Optical spectroscopy
KW - Tissue diagnostic
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U2 - 10.1117/1.3006066
DO - 10.1117/1.3006066
M3 - Article
C2 - 19123659
AN - SCOPUS:55649091264
SN - 1083-3668
VL - 13
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 6
M1 - 064012
ER -