Abstract
Conditional alleles containing LoxP recombination sites, in conjunction with Cre recombinase delivered by a variety of means, allows for spatial and temporal control of gene expression in mouse models. Here we describe a mouse strain in which a luciferase (Luc) cDNA, preceded by a LoxP-stop-LoxP (L-S-L) cassette, was introduced into the ubiquitously expressed ROSA26 locus. Mouse embryo fibroblasts derived from this strain expressed luciferase after Cre-mediated recombination in vitro. ROSA26L-S-L-Luc/+ mice expressed luciferase in a diffuse or liver-restricted pattern, as determined by non-invasive, bioluminescent imaging, when crossed to transgenic mice in which Cre was under the control of a zygotically expressed (EIIA-Cre), or a liver-restricted (albumiu-Cre), promoter, respectively. Organ-specific luciferase expression was also seen after intraparenchymal administration of an adeno-virus encoding Cre. The ROSA26 L-S-L-Luc/+ strain should be useful for characterizing Cre mouse strains and for following the fate of cells that have undergone Cre-mediated recombination in vivo.
Original language | English (US) |
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Pages (from-to) | 297-302 |
Number of pages | 6 |
Journal | Molecular imaging |
Volume | 2 |
Issue number | 4 |
DOIs | |
State | Published - Oct 2003 |
Externally published | Yes |
Keywords
- Bioluminescent
- Conditional allele
- Cre recombinase
- Knockout
- Luciferase
ASJC Scopus subject areas
- Biotechnology
- Molecular Medicine
- Biomedical Engineering
- Radiology Nuclear Medicine and imaging
- Condensed Matter Physics