TY - JOUR
T1 - mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer
AU - Lanza, Giovanni
AU - Ferracin, Manuela
AU - Gafà, Roberta
AU - Veronese, Angelo
AU - Spizzo, Riccardo
AU - Pichiorri, Flavia
AU - Liu, Chang Gong
AU - Calin, George A.
AU - Croce, Carlo M.
AU - Negrini, Massimo
PY - 2007/8/23
Y1 - 2007/8/23
N2 - Background: Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome. Results: We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H) for genome-wide expression of microRNA (miRNA) and mRNA. Based on combined miRNA and mRNA gene expression, a molecular signature consisting of twenty seven differentially expressed genes, inclusive of 8 miRNAs, could correctly distinguish MSI-H versus MSS colon cancer samples. Among the differentially expressed miRNAs, various members of the oncogenic miR-17-92 family were significantly up-regulated in MSS cancers. The majority of protein coding genes were also up-regulated in MSS cancers. Their functional classification revealed that they were most frequently associated with cell cycle, DNA replication, recombination, repair, gastrointestinal disease and immune response. Conclusion: This is the first report that indicates the existence of differences in miRNA expression between MSS versus MSI-H colorectal cancers. In addition, the work suggests that the combination of mRNA/ miRNA expression signatures may represent a general approach for improving bio-molecular classification of human cancer.
AB - Background: Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome. Results: We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H) for genome-wide expression of microRNA (miRNA) and mRNA. Based on combined miRNA and mRNA gene expression, a molecular signature consisting of twenty seven differentially expressed genes, inclusive of 8 miRNAs, could correctly distinguish MSI-H versus MSS colon cancer samples. Among the differentially expressed miRNAs, various members of the oncogenic miR-17-92 family were significantly up-regulated in MSS cancers. The majority of protein coding genes were also up-regulated in MSS cancers. Their functional classification revealed that they were most frequently associated with cell cycle, DNA replication, recombination, repair, gastrointestinal disease and immune response. Conclusion: This is the first report that indicates the existence of differences in miRNA expression between MSS versus MSI-H colorectal cancers. In addition, the work suggests that the combination of mRNA/ miRNA expression signatures may represent a general approach for improving bio-molecular classification of human cancer.
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U2 - 10.1186/1476-4598-6-54
DO - 10.1186/1476-4598-6-54
M3 - Article
C2 - 17716371
AN - SCOPUS:35748967324
SN - 1476-4598
VL - 6
JO - Molecular cancer
JF - Molecular cancer
M1 - 54
ER -