TY - JOUR
T1 - Multiple cis elements within the Igf2/H19 insulator domain organize a distance-dependent silencer. A cautionary note
AU - Ginjala, Vasudeva
AU - Holmgren, Claes
AU - Ulleras, Erik
AU - Kanduri, Chandrasekhar
AU - Pant, Vinod
AU - Lobanenkov, Victor
AU - Franklin, Gary
AU - Ohlsson, Rolf
PY - 2002/2/22
Y1 - 2002/2/22
N2 - The 5′-flank of the H19 gene harbors a differentially methylated imprinting control region that represses the maternally derived Igf2 and paternally derived H19 alleles. Here we show that the H19 imprinting control region (ICR) is a potent silencer when positioned in a promoter-proximal position. The silencing effect is not alleviated by trichostatin A treatment, suggesting that it does not involve histone deacetylase functions. When the H19 ICR is separated from the promoter by more than 1.2 ± 0.3 kb, however, trichostatin A stimulates promoter activity 10-fold. Deletion analyses revealed that the silencing feature extended throughout the ICR segment. Finally, chromatin immunopurification analyses revealed that the H19 ICR prevented trichostatin A-dependent reacetylation of histones in the promoter region in a proximal but not in a distal position. We argue that these features are likely to be side effects of the H19 ICR, rather than explaining the mechanism of silencing of the paternal H19 allele. We issue a cautionary note, therefore, that the interpretation of insulator/silencer data could be erroneous should the distance issue not be taken into consideration.
AB - The 5′-flank of the H19 gene harbors a differentially methylated imprinting control region that represses the maternally derived Igf2 and paternally derived H19 alleles. Here we show that the H19 imprinting control region (ICR) is a potent silencer when positioned in a promoter-proximal position. The silencing effect is not alleviated by trichostatin A treatment, suggesting that it does not involve histone deacetylase functions. When the H19 ICR is separated from the promoter by more than 1.2 ± 0.3 kb, however, trichostatin A stimulates promoter activity 10-fold. Deletion analyses revealed that the silencing feature extended throughout the ICR segment. Finally, chromatin immunopurification analyses revealed that the H19 ICR prevented trichostatin A-dependent reacetylation of histones in the promoter region in a proximal but not in a distal position. We argue that these features are likely to be side effects of the H19 ICR, rather than explaining the mechanism of silencing of the paternal H19 allele. We issue a cautionary note, therefore, that the interpretation of insulator/silencer data could be erroneous should the distance issue not be taken into consideration.
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U2 - 10.1074/jbc.C100552200
DO - 10.1074/jbc.C100552200
M3 - Article
C2 - 11777900
AN - SCOPUS:0037155214
SN - 0021-9258
VL - 277
SP - 5707
EP - 5710
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 8
ER -