TY - JOUR
T1 - Multiplex quantitative measurement of mrnas from fixed tissue microarray sections
AU - Armani, Michael
AU - Tangrea, Michael
AU - Yang, Brian
AU - Rosenberg, Alex
AU - Ylaya, Kris
AU - Morris, Jennifer
AU - Rodriguez-Canales, Jaime
AU - Hanson, Jeffrey
AU - Shapiro, Benjamin
AU - Emmert-Buck, Michael R.
AU - Smela, Elisabeth
AU - Hewitt, Stephen M.
PY - 2014
Y1 - 2014
N2 - The development of prognostic and diagnostic biomarkers, such as those from gene expression studies, requires independent validation in clinical specimens. Immunohistochemical analysis on tissue microarrays (TMAs) of formalin-fixed paraffin-embedded tissue is often used to increase the statistical power, and it is used more often than in situ hybridization, which can be technically limiting. Herein, we introduce a method for performing quantitative gene expression analysis across a TMA using an adaptation of 2D-RT-qPCR, a recently developed technology for measuring transcript levels in a histologic section while maintaining 2-dimensional positional information of the tissue sample. As a demonstration of utility, a TMA with tumor and normal human prostate samples was used to validate expression profiles from previous array-based gene discovery studies of prostate cancer. The results show that 2D-RT-qPCR expands the utility of TMAs to include sensitive and accurate gene expression measurements.
AB - The development of prognostic and diagnostic biomarkers, such as those from gene expression studies, requires independent validation in clinical specimens. Immunohistochemical analysis on tissue microarrays (TMAs) of formalin-fixed paraffin-embedded tissue is often used to increase the statistical power, and it is used more often than in situ hybridization, which can be technically limiting. Herein, we introduce a method for performing quantitative gene expression analysis across a TMA using an adaptation of 2D-RT-qPCR, a recently developed technology for measuring transcript levels in a histologic section while maintaining 2-dimensional positional information of the tissue sample. As a demonstration of utility, a TMA with tumor and normal human prostate samples was used to validate expression profiles from previous array-based gene discovery studies of prostate cancer. The results show that 2D-RT-qPCR expands the utility of TMAs to include sensitive and accurate gene expression measurements.
KW - Gene expression
KW - TMA
KW - Validation
KW - mRNA profiling
UR - http://www.scopus.com/inward/record.url?scp=84900496541&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84900496541&partnerID=8YFLogxK
U2 - 10.1097/PDM.0000000000000039
DO - 10.1097/PDM.0000000000000039
M3 - Review article
C2 - 24809843
AN - SCOPUS:84900496541
SN - 1541-2016
VL - 22
SP - 323
EP - 330
JO - Applied Immunohistochemistry and Molecular Morphology
JF - Applied Immunohistochemistry and Molecular Morphology
IS - 5
ER -