Multiplexed in situ immunofluorescence using dynamic DNA complexes

Ryan M. Schweller; Jan Zimak; Dzifa Y. Duose; Amina A. Qutub; Walter N. Hittelman; Michael R. Diehl

doi:10.1002/anie.201204304

Multiplexed in situ immunofluorescence using dynamic DNA complexes

Ryan M. Schweller, Jan Zimak, Dzifa Y. Duose, Amina A. Qutub, Walter N. Hittelman, Michael R. Diehl

Experimental Therapeutics

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

DNA-based erasers: The designed strand-displacement reactions between dynamic DNA probes (carrying fluorescent dyes and quencher domains and DNA-antibody conjugates generate detachable immunofluoresence-reporting complexes that can be assembled (first image) and disassembled (second image) reversibly within fixed cells. With this system, different protein targets can be imaged sequentially within the same cells (third image).

Original language	English (US)
Pages (from-to)	9292-9296
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	37
DOIs	https://doi.org/10.1002/anie.201204304
State	Published - Sep 10 2012

Keywords

DNA nanotechnology
fluorescent probes
immunochemistry
nucleic acids
strand displacement

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201204304

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AU - Hittelman, Walter N.

AU - Diehl, Michael R.

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KW - fluorescent probes

KW - immunochemistry

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KW - strand displacement

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Multiplexed in situ immunofluorescence using dynamic DNA complexes

Abstract

Keywords

ASJC Scopus subject areas

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