Multiplexed protein detection by proximity ligation for cancer biomarker validation

Simon Fredriksson; William Dixon; Hanlee Ji; Albert C. Koong; Michael Mindrinos; Ronald W. Davis

doi:10.1038/nmeth1020

Multiplexed protein detection by proximity ligation for cancer biomarker validation

Simon Fredriksson, William Dixon, Hanlee Ji, Albert C. Koong, Michael Mindrinos, Ronald W. Davis

Image Guided Cancer Therapy Research Program

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160 Scopus citations

Abstract

We present a proximity ligation-based multiplexed protein detection procedure in which several selected proteins can be detected via unique nucleic-acid identifiers and subsequently quantified by real-time PCR. The assay requires a 1-μl sample, has low-femtomolar sensitivity as well as five-log linear range and allows for modular multiplexing without cross-reactivity. The procedure can use a single polyclonal antibody batch for each target protein, simplifying affinity-reagent creation for new biomarker candidates.

Original language	English (US)
Pages (from-to)	327-329
Number of pages	3
Journal	Nature Methods
Volume	4
Issue number	4
DOIs	https://doi.org/10.1038/nmeth1020
State	Published - Apr 1 2007

ASJC Scopus subject areas

Biotechnology
Molecular Biology
Cell Biology

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10.1038/nmeth1020

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title = "Multiplexed protein detection by proximity ligation for cancer biomarker validation",

abstract = "We present a proximity ligation-based multiplexed protein detection procedure in which several selected proteins can be detected via unique nucleic-acid identifiers and subsequently quantified by real-time PCR. The assay requires a 1-μl sample, has low-femtomolar sensitivity as well as five-log linear range and allows for modular multiplexing without cross-reactivity. The procedure can use a single polyclonal antibody batch for each target protein, simplifying affinity-reagent creation for new biomarker candidates.",

author = "Simon Fredriksson and William Dixon and Hanlee Ji and Koong, {Albert C.} and Michael Mindrinos and Davis, {Ronald W.}",

note = "Funding Information: This work was supported by the Swedish Research Council, the Canary Foundation, the Liu Bie Ju Cha and Family Fellowship in Cancer and the US National Institutes of Health (Center Grant 2P01HG000205 and Glue Grant U54GM062119).",

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AU - Dixon, William

AU - Ji, Hanlee

AU - Koong, Albert C.

AU - Mindrinos, Michael

AU - Davis, Ronald W.

N1 - Funding Information: This work was supported by the Swedish Research Council, the Canary Foundation, the Liu Bie Ju Cha and Family Fellowship in Cancer and the US National Institutes of Health (Center Grant 2P01HG000205 and Glue Grant U54GM062119).

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Multiplexed protein detection by proximity ligation for cancer biomarker validation

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