Extracción de mutágenos de bilis de pacientes con patología biliar inflamatoria: prueba de Ames con uso de algodón azul.

J. C. Araya; I. Roa; H. Mano; M. Yamamoto; I. Wistuba; M. A. Villaseca; X. de Aretxabala; T. Ohta; L. Burgos

Extracción de mutágenos de bilis de pacientes con patología biliar inflamatoria: prueba de Ames con uso de algodón azul.

Translated title of the contribution: Mutagen extraction from bile of patients with inflammatory biliary pathology: Ames test using blue rayon

J. C. Araya, I. Roa, H. Mano, M. Yamamoto, I. Wistuba, M. A. Villaseca, X. de Aretxabala, T. Ohta, L. Burgos

Research output: Contribution to journal › Article › peer-review

Abstract

Gallbladder carcinoma is frequent in Chile. The aim of this study was to report the mutagenicity of whole human bile, using the Ames/Salmonella microsome assay with Salmonella typhimurium TA98. The bile of 19 patients, aged 23 to 64 years old, subjected to cholecystectomy was examined, and mutagen activity was found in 13 (72%). Mutagens were extracted using blue rayon and three dilutions for the eluted material from blue rayon were used (50, 100 and 200 ul). The best result was obtained using 200 ul. In some cases, the amount of revertive colonies was very high (over 5 times the control value). We propose that the bile from these patients possibly contains mutagenic substances with frame shift mutagenic activity and that these substances may be related to gallbladder carcinoma. Our results have addressed the importance of bile studies to elucidate the pathogenesis of gallbladder carcinoma.

Translated title of the contribution	Mutagen extraction from bile of patients with inflammatory biliary pathology: Ames test using blue rayon
Original language	Spanish
Pages (from-to)	1245-1251
Number of pages	7
Journal	Revista médica de Chile
Volume	121
Issue number	11
State	Published - Nov 1993

ASJC Scopus subject areas

General Medicine

Cite this

@article{6ee8ea2eda3d445d873ef288ce1b9a05,

title = "Extracci{\'o}n de mut{\'a}genos de bilis de pacientes con patolog{\'i}a biliar inflamatoria: prueba de Ames con uso de algod{\'o}n azul.",

abstract = "Gallbladder carcinoma is frequent in Chile. The aim of this study was to report the mutagenicity of whole human bile, using the Ames/Salmonella microsome assay with Salmonella typhimurium TA98. The bile of 19 patients, aged 23 to 64 years old, subjected to cholecystectomy was examined, and mutagen activity was found in 13 (72%). Mutagens were extracted using blue rayon and three dilutions for the eluted material from blue rayon were used (50, 100 and 200 ul). The best result was obtained using 200 ul. In some cases, the amount of revertive colonies was very high (over 5 times the control value). We propose that the bile from these patients possibly contains mutagenic substances with frame shift mutagenic activity and that these substances may be related to gallbladder carcinoma. Our results have addressed the importance of bile studies to elucidate the pathogenesis of gallbladder carcinoma.",

author = "Araya, {J. C.} and I. Roa and H. Mano and M. Yamamoto and I. Wistuba and Villaseca, {M. A.} and {de Aretxabala}, X. and T. Ohta and L. Burgos",

year = "1993",

month = nov,

language = "Spanish",

volume = "121",

pages = "1245--1251",

journal = "Revista m{\'e}dica de Chile",

issn = "0034-9887",

publisher = "Sociedad Medica de Santiago",

number = "11",

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TY - JOUR

T1 - Extracción de mutágenos de bilis de pacientes con patología biliar inflamatoria

T2 - prueba de Ames con uso de algodón azul.

AU - Araya, J. C.

AU - Roa, I.

AU - Mano, H.

AU - Yamamoto, M.

AU - Wistuba, I.

AU - Villaseca, M. A.

AU - de Aretxabala, X.

AU - Ohta, T.

AU - Burgos, L.

PY - 1993/11

Y1 - 1993/11

N2 - Gallbladder carcinoma is frequent in Chile. The aim of this study was to report the mutagenicity of whole human bile, using the Ames/Salmonella microsome assay with Salmonella typhimurium TA98. The bile of 19 patients, aged 23 to 64 years old, subjected to cholecystectomy was examined, and mutagen activity was found in 13 (72%). Mutagens were extracted using blue rayon and three dilutions for the eluted material from blue rayon were used (50, 100 and 200 ul). The best result was obtained using 200 ul. In some cases, the amount of revertive colonies was very high (over 5 times the control value). We propose that the bile from these patients possibly contains mutagenic substances with frame shift mutagenic activity and that these substances may be related to gallbladder carcinoma. Our results have addressed the importance of bile studies to elucidate the pathogenesis of gallbladder carcinoma.

AB - Gallbladder carcinoma is frequent in Chile. The aim of this study was to report the mutagenicity of whole human bile, using the Ames/Salmonella microsome assay with Salmonella typhimurium TA98. The bile of 19 patients, aged 23 to 64 years old, subjected to cholecystectomy was examined, and mutagen activity was found in 13 (72%). Mutagens were extracted using blue rayon and three dilutions for the eluted material from blue rayon were used (50, 100 and 200 ul). The best result was obtained using 200 ul. In some cases, the amount of revertive colonies was very high (over 5 times the control value). We propose that the bile from these patients possibly contains mutagenic substances with frame shift mutagenic activity and that these substances may be related to gallbladder carcinoma. Our results have addressed the importance of bile studies to elucidate the pathogenesis of gallbladder carcinoma.

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AN - SCOPUS:0027697505

SN - 0034-9887

VL - 121

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EP - 1251

JO - Revista médica de Chile

JF - Revista médica de Chile

IS - 11

ER -

Extracción de mutágenos de bilis de pacientes con patología biliar inflamatoria: prueba de Ames con uso de algodón azul.

Abstract

ASJC Scopus subject areas

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