Mutation discovery in bacterial genomes: Metronidazole resistance in Helicobacter pylori

Thomas J. Albert; Daiva Dailidiene; Giedrius Dailide; Jason E. Norton; Awdhesh Kalia; Todd A. Richmond; Michael Molla; Jaz Singh; Roland D. Green; Douglas E. Berg

doi:10.1038/nmeth805

Mutation discovery in bacterial genomes: Metronidazole resistance in Helicobacter pylori

Thomas J. Albert, Daiva Dailidiene, Giedrius Dailide, Jason E. Norton, Awdhesh Kalia, Todd A. Richmond, Michael Molla, Jaz Singh, Roland D. Green, Douglas E. Berg

Research output: Contribution to journal › Article › peer-review

157 Scopus citations

Abstract

We developed a microarray hybridization-based method, 'comparative genome sequencing' (CGS), to find mutations in bacterial genomes and used it to study metronidazole resistance in H. pylori. CGS identified mutations in several genes, most likely affecting metronidazole activation, and produced no false positives in analysis of three megabases. We conclude that CGS identifies mutations in bacterial genomes efficiently, should enrich understanding of systems biology and genome evolution, and help track pathogens during outbreaks.

Original language	English (US)
Pages (from-to)	951-953
Number of pages	3
Journal	Nature Methods
Volume	2
Issue number	12
DOIs	https://doi.org/10.1038/nmeth805
State	Published - Dec 2005
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth805

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title = "Mutation discovery in bacterial genomes: Metronidazole resistance in Helicobacter pylori",

abstract = "We developed a microarray hybridization-based method, 'comparative genome sequencing' (CGS), to find mutations in bacterial genomes and used it to study metronidazole resistance in H. pylori. CGS identified mutations in several genes, most likely affecting metronidazole activation, and produced no false positives in analysis of three megabases. We conclude that CGS identifies mutations in bacterial genomes efficiently, should enrich understanding of systems biology and genome evolution, and help track pathogens during outbreaks.",

author = "Albert, {Thomas J.} and Daiva Dailidiene and Giedrius Dailide and Norton, {Jason E.} and Awdhesh Kalia and Richmond, {Todd A.} and Michael Molla and Jaz Singh and Green, {Roland D.} and Berg, {Douglas E.}",

note = "Funding Information: We thank P. Hoffman, X. Pan and S. Weissman for stimulating discussions. This research was supported by the US National Institutes of Health grants RO1 AI38166, RO1 DK63041, R21 AI057713 and P30 DK52574.",

year = "2005",

month = dec,

doi = "10.1038/nmeth805",

language = "English (US)",

volume = "2",

pages = "951--953",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Public Library of Science",

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TY - JOUR

T1 - Mutation discovery in bacterial genomes

T2 - Metronidazole resistance in Helicobacter pylori

AU - Albert, Thomas J.

AU - Dailidiene, Daiva

AU - Dailide, Giedrius

AU - Norton, Jason E.

AU - Kalia, Awdhesh

AU - Richmond, Todd A.

AU - Molla, Michael

AU - Singh, Jaz

AU - Green, Roland D.

AU - Berg, Douglas E.

N1 - Funding Information: We thank P. Hoffman, X. Pan and S. Weissman for stimulating discussions. This research was supported by the US National Institutes of Health grants RO1 AI38166, RO1 DK63041, R21 AI057713 and P30 DK52574.

PY - 2005/12

Y1 - 2005/12

N2 - We developed a microarray hybridization-based method, 'comparative genome sequencing' (CGS), to find mutations in bacterial genomes and used it to study metronidazole resistance in H. pylori. CGS identified mutations in several genes, most likely affecting metronidazole activation, and produced no false positives in analysis of three megabases. We conclude that CGS identifies mutations in bacterial genomes efficiently, should enrich understanding of systems biology and genome evolution, and help track pathogens during outbreaks.

AB - We developed a microarray hybridization-based method, 'comparative genome sequencing' (CGS), to find mutations in bacterial genomes and used it to study metronidazole resistance in H. pylori. CGS identified mutations in several genes, most likely affecting metronidazole activation, and produced no false positives in analysis of three megabases. We conclude that CGS identifies mutations in bacterial genomes efficiently, should enrich understanding of systems biology and genome evolution, and help track pathogens during outbreaks.

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Mutation discovery in bacterial genomes: Metronidazole resistance in Helicobacter pylori

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