TY - JOUR
T1 - Mutation in a calpain-like protease affects the posttranslational mannosylation of phosphatases in Aspergillus nidulans
AU - Nozawa, S. R.
AU - May, G. S.
AU - Martinez-Rossi, N. M.
AU - Ferreira-Nozawa, M. S.
AU - Coutinho-Netto, J.
AU - Maccheroni, W.
AU - Rossi, A.
N1 - Funding Information:
This research was supported by grants from FAPESP and CNPq (Brazil) and by NIH (GM53027) and NSF (MCB-9513382) (G.S.M., USA). S.R.N and M.S.F.-N. were supported by postgraduate student fellowships from FAPESP and W.M. Jr. by a postdoctoral student fellowship from FAPESP. We thank Mércia V. Carlos (HPLC analysis, CAPES/CNPq agreement), José Seminate-Filho, and Newton R. Alves for their technical assistance.
PY - 2003/3
Y1 - 2003/3
N2 - In this communication, we show that the palB7 mutation drastically reduced the mannose and N-acetylgalactosamine content of the pacA-encoded acid phosphatase secreted by the fungus Aspergillus nidulans at pH 5.0, compared to a control strain. By using mRNA differential display reverse transcription and polymerase chain reaction, we isolated two cDNAs from the control pabaA1 strain that were not detected in the palB7 mutant strain that encode a mannosyl transferase and a NADH-ubiquinone oxidoreductase. Thus, a defect in the posttranslational mannosylation of proteins could be the consequence of mutations in the palB gene, which encodes for a nuclear calpain-like protease that may have specific functions in the processing of transcription factor(s) similar to its homolog, RIM13, in Saccharomyces cereviseae.
AB - In this communication, we show that the palB7 mutation drastically reduced the mannose and N-acetylgalactosamine content of the pacA-encoded acid phosphatase secreted by the fungus Aspergillus nidulans at pH 5.0, compared to a control strain. By using mRNA differential display reverse transcription and polymerase chain reaction, we isolated two cDNAs from the control pabaA1 strain that were not detected in the palB7 mutant strain that encode a mannosyl transferase and a NADH-ubiquinone oxidoreductase. Thus, a defect in the posttranslational mannosylation of proteins could be the consequence of mutations in the palB gene, which encodes for a nuclear calpain-like protease that may have specific functions in the processing of transcription factor(s) similar to its homolog, RIM13, in Saccharomyces cereviseae.
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U2 - 10.1016/S1087-1845(02)00521-2
DO - 10.1016/S1087-1845(02)00521-2
M3 - Article
C2 - 12620258
AN - SCOPUS:0344406973
SN - 1087-1845
VL - 38
SP - 220
EP - 227
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
IS - 2
ER -