TY - JOUR
T1 - Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective
AU - Kyrish, Matthew
AU - Dobbs, Jessica
AU - Jain, Shalini
AU - Wang, Xiao
AU - Yu, Dihua
AU - Richards-Kortum, Rebecca
AU - Tkaczyk, Tomasz S.
N1 - Funding Information:
Funding was provided by NIH grants R01 EB007594, R01 CA103830, and R01 CA124319 and by the Susan G. Komen Promise Grant KG091020.
PY - 2013
Y1 - 2013
N2 - In order to diagnose cancer, a sample must be removed, prepared, and examined under a microscope, which is expensive, invasive, and time consuming. Fiber optic fluorescence endomicroscopy, where an image guide is used to obtain high-resolution images of tissue in vivo, has shown promise as an alternative to conventional biopsies. However, the resolution of standard endomicroscopy is limited by the fiber bundle sampling frequency and out-of-focus light. A system is presented which incorporates a plastic, achromatic objective to increase the sampling and which provides optical sectioning via structured illumination to reject background light. An image is relayed from the sample by a fiber bundle with the custom 2.1-mm outer diameter objective lens integrated to the distal tip. The objective is corrected for the excitation and the emission wavelengths of proflavine (452 and 515 nm). It magnifies the object onto the fiber bundle to improve the system's lateral resolution by increasing the sampling. The plastic lenses were fabricated via single-point diamond turning and assembled using a zero alignment technique. Ex vivo images of normal and neoplastic murine mammary tissues stained with proflavine are captured. The system achieves higher contrast and resolves smaller features than standard fluorescence endomicroscopy.
AB - In order to diagnose cancer, a sample must be removed, prepared, and examined under a microscope, which is expensive, invasive, and time consuming. Fiber optic fluorescence endomicroscopy, where an image guide is used to obtain high-resolution images of tissue in vivo, has shown promise as an alternative to conventional biopsies. However, the resolution of standard endomicroscopy is limited by the fiber bundle sampling frequency and out-of-focus light. A system is presented which incorporates a plastic, achromatic objective to increase the sampling and which provides optical sectioning via structured illumination to reject background light. An image is relayed from the sample by a fiber bundle with the custom 2.1-mm outer diameter objective lens integrated to the distal tip. The objective is corrected for the excitation and the emission wavelengths of proflavine (452 and 515 nm). It magnifies the object onto the fiber bundle to improve the system's lateral resolution by increasing the sampling. The plastic lenses were fabricated via single-point diamond turning and assembled using a zero alignment technique. Ex vivo images of normal and neoplastic murine mammary tissues stained with proflavine are captured. The system achieves higher contrast and resolves smaller features than standard fluorescence endomicroscopy.
KW - Endomicroscope
KW - achromatic objective
KW - cancer diagnosis
KW - fluorescence
KW - precision machining
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U2 - 10.1117/1.JBO.18.9.096003
DO - 10.1117/1.JBO.18.9.096003
M3 - Article
C2 - 24002190
AN - SCOPUS:84887949402
SN - 1083-3668
VL - 18
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 9
M1 - 096003
ER -