Nerve growth factor induces mast cell degranulation without changing intracellular calcium levels

N. Mazurek; G. Weskamp; P. Erne; U. Otten

doi:10.1016/0014-5793(86)80428-8

Nerve growth factor induces mast cell degranulation without changing intracellular calcium levels

N. Mazurek, G. Weskamp, P. Erne, U. Otten

Gastroenterology Hepatology & Nutrition

Research output: Contribution to journal › Article › peer-review

123 Scopus citations

Abstract

Nerve growth factor (NGF) induces degranulation of rat peritoneal mast cells (RPMC) in a dose-dependent manner, providing direct evidence for its action on non-neuronal tissues. Activation of RPMC by NGF depends on lysophosphatidylserine and extracellular calcium. NGF-mediated RPMC degranulation is not coupled to a transient increase in intracellular free calcium ([Ca²⁺]). It is suggested that NGF has a unique mode of action independent of ([Ca²⁺])_i and presumably also without involving protein kinase C activation as indicated by the effects of phorbol esters and NGF on antigen-evoked [Ca²⁺]_i rise.

Original language	English (US)
Pages (from-to)	315-320
Number of pages	6
Journal	FEBS Letters
Volume	198
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(86)80428-8
State	Published - Mar 31 1986

Keywords

(Rat peritoneal mast cell, Rat leukemia cell line)
12-O-Tetradecanoylphorbol 13-acetate
Ca
Degranulation
Nerve growth factor

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(86)80428-8

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title = "Nerve growth factor induces mast cell degranulation without changing intracellular calcium levels",

abstract = "Nerve growth factor (NGF) induces degranulation of rat peritoneal mast cells (RPMC) in a dose-dependent manner, providing direct evidence for its action on non-neuronal tissues. Activation of RPMC by NGF depends on lysophosphatidylserine and extracellular calcium. NGF-mediated RPMC degranulation is not coupled to a transient increase in intracellular free calcium ([Ca2+]). It is suggested that NGF has a unique mode of action independent of ([Ca2+])i and presumably also without involving protein kinase C activation as indicated by the effects of phorbol esters and NGF on antigen-evoked [Ca2+]i rise.",

keywords = "(Rat peritoneal mast cell, Rat leukemia cell line), 12-O-Tetradecanoylphorbol 13-acetate, Ca, Degranulation, Nerve growth factor",

author = "N. Mazurek and G. Weskamp and P. Erne and U. Otten",

note = "Funding Information: This work was supported by grant 3344-082 from the Swiss National Foundation. N.M. has beens upportedb y a long-termf ellowshipf rom the European Molecular Biology Organization, and P.E. by the Swiss Foundation for Biological and Medical Fellowship.",

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doi = "10.1016/0014-5793(86)80428-8",

language = "English (US)",

volume = "198",

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T1 - Nerve growth factor induces mast cell degranulation without changing intracellular calcium levels

AU - Mazurek, N.

AU - Weskamp, G.

AU - Erne, P.

AU - Otten, U.

N1 - Funding Information: This work was supported by grant 3344-082 from the Swiss National Foundation. N.M. has beens upportedb y a long-termf ellowshipf rom the European Molecular Biology Organization, and P.E. by the Swiss Foundation for Biological and Medical Fellowship.

PY - 1986/3/31

Y1 - 1986/3/31

N2 - Nerve growth factor (NGF) induces degranulation of rat peritoneal mast cells (RPMC) in a dose-dependent manner, providing direct evidence for its action on non-neuronal tissues. Activation of RPMC by NGF depends on lysophosphatidylserine and extracellular calcium. NGF-mediated RPMC degranulation is not coupled to a transient increase in intracellular free calcium ([Ca2+]). It is suggested that NGF has a unique mode of action independent of ([Ca2+])i and presumably also without involving protein kinase C activation as indicated by the effects of phorbol esters and NGF on antigen-evoked [Ca2+]i rise.

AB - Nerve growth factor (NGF) induces degranulation of rat peritoneal mast cells (RPMC) in a dose-dependent manner, providing direct evidence for its action on non-neuronal tissues. Activation of RPMC by NGF depends on lysophosphatidylserine and extracellular calcium. NGF-mediated RPMC degranulation is not coupled to a transient increase in intracellular free calcium ([Ca2+]). It is suggested that NGF has a unique mode of action independent of ([Ca2+])i and presumably also without involving protein kinase C activation as indicated by the effects of phorbol esters and NGF on antigen-evoked [Ca2+]i rise.

KW - (Rat peritoneal mast cell, Rat leukemia cell line)

KW - 12-O-Tetradecanoylphorbol 13-acetate

KW - Ca

KW - Degranulation

KW - Nerve growth factor

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JO - FEBS Letters

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ER -

Nerve growth factor induces mast cell degranulation without changing intracellular calcium levels

Abstract

Keywords

ASJC Scopus subject areas

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