TY - JOUR
T1 - Nestin and NG2 transgenes reveal two populations of perivascular cells stimulated by photobiomodulation
AU - Gomes, Natália A.
AU - do Valle, Isabella B.
AU - Gleber-Netto, Frederico O.
AU - Silva, Tarcília A.
AU - Oliveira, Hortência M.de Castro
AU - de Oliveira, Rafaela F.
AU - Ferreira, Luiza de Almeida Q.
AU - Castilho, Lia S.
AU - Reis, Paulo H.R.G.
AU - Prazeres, Pedro H.D.M.
AU - Menezes, Gustavo B.
AU - de Magalhães, Cláudia S.
AU - Mesquita, Ricardo A.
AU - Marques, Márcia M.
AU - Birbrair, Alexander
AU - Diniz, Ivana M.A.
N1 - Funding Information:
Part of the illustrative icons used for Figure S1 is the intellectual property of LES LABORATOIRES SERVIER, SAS (https://smart.servier.com) or a gift from Flávio Moura Rezende Filho. We are grateful for the technical support of Domenico Romani and Mara Maia in sample processing at the School of Dentistry (UFMG). We thank the OhLab for the support in 3D printing. Finally, the Centro de Aquisição e Processamento de Imagens—CAPI (https://www2.icb.ufmg.br/capi/), the Center for Gastrointestinal Biology at UFMG and Fundação Oswaldo Cruz (René Rachou Institute) are acknowledged for providing the equipment and technical support for experiments involving image acquisition. This study was supported in part by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; Grant 438748/2018-2 to Prof. Diniz), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG; Grant APQ 0100-18 to Prof. Diniz), and Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES scholarships, Finance code 001). Alexander Birbrair is supported by a grant from Instituto Serrapilheira: Serra-1708-15285.
Funding Information:
Part of the illustrative icons used for Figure S1 is the intellectual property of LES LABORATOIRES SERVIER, SAS ( https://smart.servier.com ) or a gift from Flávio Moura Rezende Filho. We are grateful for the technical support of Domenico Romani and Mara Maia in sample processing at the School of Dentistry (UFMG). We thank the OhLab for the support in 3D printing. Finally, the Centro de Aquisição e Processamento de Imagens—CAPI ( https://www2.icb.ufmg.br/capi/ ), the Center for Gastrointestinal Biology at UFMG and Fundação Oswaldo Cruz (René Rachou Institute) are acknowledged for providing the equipment and technical support for experiments involving image acquisition. This study was supported in part by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; Grant 438748/2018‐2 to Prof. Diniz), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG; Grant APQ 0100‐18 to Prof. Diniz), and Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES scholarships, Finance code 001).
Publisher Copyright:
© 2022 Wiley Periodicals LLC.
PY - 2022/4
Y1 - 2022/4
N2 - Pericytes and glial cells are known to collaborate in dental pulp tissue repair. Cell-based therapies that stimulate these stromal components may be of therapeutic relevance for partially vital dental pulp conditions. This study aimed to examine the early effect of photobiomodulation (PBM) in pericytes from experimentally injured pulp tissue. To accomplish this, we used the Nestin-GFP/NG2-DsRed mice, which could allow the identification of distinct pericyte phenotypes. We discovered the presence of two pericytes subsets within the dental pulp, the Nestin +NG2+ (type-2) and Nestin−NG2+ (type-1). Upon injury, PBM treatment led to a significant increase in Nestin+ cells and pericytes. This boost was mainly conferred by the more committed pericyte subset (NestinNG2+). PBM also stimulated terminal blood vessels sprouting adjacent to the injury site while maintaining signs of pulp vitality. In vitro, PBM induced VEGF upregulation, improved dental pulp cells proliferation and migration, and favored their mineralization potential. Herein, different subsets of perivascular cells were unveiled in the pulp tissue. PBM enhanced not only NG2+ cells but nestin-expressing progenitors in the injured dental pulp.
AB - Pericytes and glial cells are known to collaborate in dental pulp tissue repair. Cell-based therapies that stimulate these stromal components may be of therapeutic relevance for partially vital dental pulp conditions. This study aimed to examine the early effect of photobiomodulation (PBM) in pericytes from experimentally injured pulp tissue. To accomplish this, we used the Nestin-GFP/NG2-DsRed mice, which could allow the identification of distinct pericyte phenotypes. We discovered the presence of two pericytes subsets within the dental pulp, the Nestin +NG2+ (type-2) and Nestin−NG2+ (type-1). Upon injury, PBM treatment led to a significant increase in Nestin+ cells and pericytes. This boost was mainly conferred by the more committed pericyte subset (NestinNG2+). PBM also stimulated terminal blood vessels sprouting adjacent to the injury site while maintaining signs of pulp vitality. In vitro, PBM induced VEGF upregulation, improved dental pulp cells proliferation and migration, and favored their mineralization potential. Herein, different subsets of perivascular cells were unveiled in the pulp tissue. PBM enhanced not only NG2+ cells but nestin-expressing progenitors in the injured dental pulp.
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U2 - 10.1002/jcp.30680
DO - 10.1002/jcp.30680
M3 - Article
C2 - 35040139
AN - SCOPUS:85122891882
SN - 0021-9541
VL - 237
SP - 2198
EP - 2210
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 4
ER -