TY - JOUR
T1 - Next-generation sequencing for the general cancer patient
AU - Avila, Monica
AU - Meric-Bernstam, Funda
N1 - Funding Information:
This work was supported in part by NIH Research Training Grant T32 CA101642 (to Dr Avila), The Cancer Prevention and Research Institute of Texas (RP150535 to Dr Meric-Bernstam), the Sheikh Khalifa Bin Zayed Al Nahyan Institute for Personalized Cancer Therapy (to Dr Meric-Bernstam), and the MD Anderson Cancer Center Support Grant (P30 CA016672 to Dr Meric-Bernstam).
Publisher Copyright:
© 2019, Millennium Medical Publishing, Inc.. All rights reserved.
PY - 2019/8
Y1 - 2019/8
N2 - Next-generation sequencing is a novel method of DNA sequencing that has become a cornerstone of precision oncology. This sequencing method detects differences in specific DNA sequences between a sample and a reference genome or matched normal DNA. In addition to single-nucleotide variants, other insertions, deletions, copy number changes, and fusions may be drivers of cancer growth, and thus represent therapeutic opportunities. As a result, genomic characterization has been increasingly used to guide treatment decisions, especially in patients with advanced disease. This review discusses the basic technologies involved in next-generation sequencing, the applications of this method, and limitations in the clinical realm.
AB - Next-generation sequencing is a novel method of DNA sequencing that has become a cornerstone of precision oncology. This sequencing method detects differences in specific DNA sequences between a sample and a reference genome or matched normal DNA. In addition to single-nucleotide variants, other insertions, deletions, copy number changes, and fusions may be drivers of cancer growth, and thus represent therapeutic opportunities. As a result, genomic characterization has been increasingly used to guide treatment decisions, especially in patients with advanced disease. This review discusses the basic technologies involved in next-generation sequencing, the applications of this method, and limitations in the clinical realm.
KW - Genetics
KW - Genomics
KW - Next-generation sequencing
KW - Precision oncology
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M3 - Article
C2 - 31449513
AN - SCOPUS:85071390940
SN - 1543-0790
VL - 17
SP - 447
EP - 454
JO - Clinical Advances in Hematology and Oncology
JF - Clinical Advances in Hematology and Oncology
IS - 8
ER -