Nitric oxide protects endothelium from cadmium mediated leakiness

Shunmugam Nagarajan; Saranya Rajendran; Uttara Saran; M. Krishna Priya; Akila Swaminathan; Jamila H. Siamwala; Swaraj Sinha; Vimal Veeriah; Punam Sonar; Vivek Jadhav; B. M. Jaffar Ali; Suvro Chatterjee

doi:10.1002/cbin.10070

Nitric oxide protects endothelium from cadmium mediated leakiness

Shunmugam Nagarajan, Saranya Rajendran, Uttara Saran, M. Krishna Priya, Akila Swaminathan, Jamila H. Siamwala, Swaraj Sinha, Vimal Veeriah, Punam Sonar, Vivek Jadhav, B. M. Jaffar Ali, Suvro Chatterjee

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Cadmium targets the vascular endothelium causing endothelial dysfunction and leakiness of endothelial barrier. Nitric oxide plays a major role in mediating endothelial functions including angiogenesis, migration and permeability. The present study investigates the nitric oxide effects on cadmium induced endothelial leakiness. Results of ex vivo and in vitro permeability assays showed that even a sub-lethal dose of cadmium chloride (1 μM) was sufficient to induce leakiness of endothelial cells. Cadmium drastically altered the actin polymerisation pattern and membrane tension of these cells compared to controls. Addition of nitric oxide donor Spermine NONOate (SP) significantly blunted cadmium-mediated effects and recover endothelial cells integrity. Cadmium-induced cytoskeletal rearrangements and membrane leakiness are associated with the low nitric oxide availability and high reactive oxygen species generation. In brief, we show the protective role of nitric oxide against cadmium-mediated endothelial leakiness.

Original language	English (US)
Pages (from-to)	495-506
Number of pages	12
Journal	Cell Biology International
Volume	37
Issue number	5
DOIs	https://doi.org/10.1002/cbin.10070
State	Published - May 2013
Externally published	Yes

Keywords

Cadmium
Cytoskeleton
Endothelial cells
Nitric oxide
Nitric oxide donor

ASJC Scopus subject areas

Cell Biology

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10.1002/cbin.10070

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title = "Nitric oxide protects endothelium from cadmium mediated leakiness",

abstract = "Cadmium targets the vascular endothelium causing endothelial dysfunction and leakiness of endothelial barrier. Nitric oxide plays a major role in mediating endothelial functions including angiogenesis, migration and permeability. The present study investigates the nitric oxide effects on cadmium induced endothelial leakiness. Results of ex vivo and in vitro permeability assays showed that even a sub-lethal dose of cadmium chloride (1 μM) was sufficient to induce leakiness of endothelial cells. Cadmium drastically altered the actin polymerisation pattern and membrane tension of these cells compared to controls. Addition of nitric oxide donor Spermine NONOate (SP) significantly blunted cadmium-mediated effects and recover endothelial cells integrity. Cadmium-induced cytoskeletal rearrangements and membrane leakiness are associated with the low nitric oxide availability and high reactive oxygen species generation. In brief, we show the protective role of nitric oxide against cadmium-mediated endothelial leakiness.",

keywords = "Cadmium, Cytoskeleton, Endothelial cells, Nitric oxide, Nitric oxide donor",

author = "Shunmugam Nagarajan and Saranya Rajendran and Uttara Saran and {Krishna Priya}, M. and Akila Swaminathan and Siamwala, {Jamila H.} and Swaraj Sinha and Vimal Veeriah and Punam Sonar and Vivek Jadhav and {Jaffar Ali}, {B. M.} and Suvro Chatterjee",

year = "2013",

month = may,

doi = "10.1002/cbin.10070",

language = "English (US)",

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journal = "Cell Biology International",

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AU - Nagarajan, Shunmugam

AU - Rajendran, Saranya

AU - Saran, Uttara

AU - Krishna Priya, M.

AU - Swaminathan, Akila

AU - Siamwala, Jamila H.

AU - Sinha, Swaraj

AU - Veeriah, Vimal

AU - Sonar, Punam

AU - Jadhav, Vivek

AU - Jaffar Ali, B. M.

AU - Chatterjee, Suvro

PY - 2013/5

Y1 - 2013/5

N2 - Cadmium targets the vascular endothelium causing endothelial dysfunction and leakiness of endothelial barrier. Nitric oxide plays a major role in mediating endothelial functions including angiogenesis, migration and permeability. The present study investigates the nitric oxide effects on cadmium induced endothelial leakiness. Results of ex vivo and in vitro permeability assays showed that even a sub-lethal dose of cadmium chloride (1 μM) was sufficient to induce leakiness of endothelial cells. Cadmium drastically altered the actin polymerisation pattern and membrane tension of these cells compared to controls. Addition of nitric oxide donor Spermine NONOate (SP) significantly blunted cadmium-mediated effects and recover endothelial cells integrity. Cadmium-induced cytoskeletal rearrangements and membrane leakiness are associated with the low nitric oxide availability and high reactive oxygen species generation. In brief, we show the protective role of nitric oxide against cadmium-mediated endothelial leakiness.

AB - Cadmium targets the vascular endothelium causing endothelial dysfunction and leakiness of endothelial barrier. Nitric oxide plays a major role in mediating endothelial functions including angiogenesis, migration and permeability. The present study investigates the nitric oxide effects on cadmium induced endothelial leakiness. Results of ex vivo and in vitro permeability assays showed that even a sub-lethal dose of cadmium chloride (1 μM) was sufficient to induce leakiness of endothelial cells. Cadmium drastically altered the actin polymerisation pattern and membrane tension of these cells compared to controls. Addition of nitric oxide donor Spermine NONOate (SP) significantly blunted cadmium-mediated effects and recover endothelial cells integrity. Cadmium-induced cytoskeletal rearrangements and membrane leakiness are associated with the low nitric oxide availability and high reactive oxygen species generation. In brief, we show the protective role of nitric oxide against cadmium-mediated endothelial leakiness.

KW - Cadmium

KW - Cytoskeleton

KW - Endothelial cells

KW - Nitric oxide

KW - Nitric oxide donor

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Nitric oxide protects endothelium from cadmium mediated leakiness

Abstract

Keywords

ASJC Scopus subject areas

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