Abstract
Intact cells of the denitrifying fungus Fusarium oxysporum were previously shown to catalyze codenitrification to form a hybrid nitrous oxide (N 2O) species from nitrite and other nitrogen compounds such as azide and ammonia. Here we show that cytochrome P450nor can catalyze the codenitrification reaction to form N2O from nitric oxide (NO) but not nitrite, and azide or ammonia. The results show that the direct substrate of the codenitrification by intact cells should not be nitrite but NO, which is formed from nitrite by the reaction of a dissimilatory nitrite reductase.
Original language | English (US) |
---|---|
Pages (from-to) | 473-475 |
Number of pages | 3 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 68 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2004 |
Externally published | Yes |
Keywords
- Codenitrification
- Cytochrome P450nor
- P450nor
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry