TY - JOUR
T1 - Novel antigens in non-small cell lung cancer
T2 - SP17, AKAP4, and PTTG1 are potential immunotherapeutic targets
AU - Mirandola, Leonardo
AU - Figueroa, Jose A.
AU - Phan, Tam T.
AU - Grizzi, Fabio
AU - Kim, Minji
AU - Rahman, Rakhshanda Layeequr
AU - Jenkins, Marjorie R.
AU - Cobos, Everardo
AU - Jumper, Cynthia
AU - Alalawi, Raed
AU - Chiriva-Internati, Maurizio
PY - 2015
Y1 - 2015
N2 - Lung cancer is the leading cause of cancer deaths in both genders worldwide, with an incidence only second to prostate cancer in men and breast cancer in women. The lethality of the disease highlights the urgent need for innovative therapeutic options. Immunotherapy can afford efficient and specific targeting of tumor cells, improving efficacy and reducing the side effects of current therapies. We have previously reported the aberrant expression of cancer/testis antigens (CTAs) in tumors of unrelated histological origin. In this study we investigated the expression and immunogenicity of the CTAs, Sperm Protein 17 (SP17), A-kinase anchor protein 4 (AKAP4) and Pituitary Tumor Transforming Gene 1 (PTTG1) in human non-small cell lung cancer (NSCLC) cell lines and primary tumors. We found that SP17, AKAP4 and PTTG1 are aberrantly expressed in cancer samples, compared to normal lung cell lines and tissues. We established the immunogenicity of these CTAs by measuring CTA-specific autoantibodies in patients' sera and generating CTA-specific autologous cytotoxic lymphocytes from patients' peripheral blood mononuclear cells. Our results provide proof of principle that the CTAs SP17/AKAP4/PTTG1 are expressed in both human NSCLC cell lines and primary tumors and can elicit an immunogenic response in lung cancer patients.
AB - Lung cancer is the leading cause of cancer deaths in both genders worldwide, with an incidence only second to prostate cancer in men and breast cancer in women. The lethality of the disease highlights the urgent need for innovative therapeutic options. Immunotherapy can afford efficient and specific targeting of tumor cells, improving efficacy and reducing the side effects of current therapies. We have previously reported the aberrant expression of cancer/testis antigens (CTAs) in tumors of unrelated histological origin. In this study we investigated the expression and immunogenicity of the CTAs, Sperm Protein 17 (SP17), A-kinase anchor protein 4 (AKAP4) and Pituitary Tumor Transforming Gene 1 (PTTG1) in human non-small cell lung cancer (NSCLC) cell lines and primary tumors. We found that SP17, AKAP4 and PTTG1 are aberrantly expressed in cancer samples, compared to normal lung cell lines and tissues. We established the immunogenicity of these CTAs by measuring CTA-specific autoantibodies in patients' sera and generating CTA-specific autologous cytotoxic lymphocytes from patients' peripheral blood mononuclear cells. Our results provide proof of principle that the CTAs SP17/AKAP4/PTTG1 are expressed in both human NSCLC cell lines and primary tumors and can elicit an immunogenic response in lung cancer patients.
KW - Cancer vaccines
KW - Cancer/testis antigens
KW - Lung cancer
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UR - http://www.scopus.com/inward/citedby.url?scp=84923296203&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.2802
DO - 10.18632/oncotarget.2802
M3 - Article
C2 - 25739119
AN - SCOPUS:84923296203
SN - 1949-2553
VL - 6
SP - 2812
EP - 2826
JO - Oncotarget
JF - Oncotarget
IS - 5
ER -