Abstract
Incubation of HeLa cells with the IMP dehydrogenase inhibitors: ribavirin (100 μM, 4 h), tiazofurin (100 μM, 4 h), selenazofurin (100 μM, 4 h), or mycophenolic acid (10 μM, 4 h) resulted in ∼70% reduction in cellular GTP pools and shifting of nucleophosmin/B23 from nucleoli to nucleoplasm as detected by immunofluorescence (B23-translocation). Enzyme-linked immunosorbent assay and Western blot assay showed there is no loss or degradation of nucleophosmin/B23 protein during drug treatment. This translocation effect could be prevented by co-incubation with guanosine (100 μM) or reversed by addition of guanosine (100 μM) to the culture medium after B23-translocation had been induced by these inhibitors. Under these conditions of guanosine supplementation, cellular GTP pool concentrations were maintained at the control level. These results indicate that localization of nucleophosmin/B23 into the nucleolus is dependent on the cellular GTP level.
Original language | English (US) |
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Pages (from-to) | 5823-5827 |
Number of pages | 5 |
Journal | Journal of Biological Chemistry |
Volume | 268 |
Issue number | 8 |
State | Published - Mar 15 1993 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology