TY - JOUR
T1 - Nucleolin promotes in vitro translation of feline calicivirus genomic RNA
AU - Hernández, Beatriz Alvarado
AU - Sandoval-Jaime, Carlos
AU - Sosnovtsev, Stanislav V.
AU - Green, Kim Y.
AU - Gutiérrez-Escolano, Ana Lorena
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Feline calicivirus depends on host-cell proteins for its replication. We previously showed that knockdown of nucleolin (NCL), a phosphoprotein involved in ribosome biogenesis, resulted in the reduction of FCV protein synthesis and virus yield. Here, we found that NCL may not be involved in FCV binding and entry into cells, but it binds to both ends of the FCV genomic RNA, and stimulates its translation in vitro. AGRO100, an aptamer that specifically binds and inactivates NCL, caused a strong reduction in FCV protein synthesis. This effect could be reversed by the addition of full-length NCL but not by a δrNCL, lacking the N-terminal domain. Consistent with this, FCV infection of CrFK cells stably expressing δrNCL led to a reduction in virus protein translation. These results suggest that NCL is part of the FCV RNA translational complex, and that the N-terminal part of the protein is required for efficient FCV replication.
AB - Feline calicivirus depends on host-cell proteins for its replication. We previously showed that knockdown of nucleolin (NCL), a phosphoprotein involved in ribosome biogenesis, resulted in the reduction of FCV protein synthesis and virus yield. Here, we found that NCL may not be involved in FCV binding and entry into cells, but it binds to both ends of the FCV genomic RNA, and stimulates its translation in vitro. AGRO100, an aptamer that specifically binds and inactivates NCL, caused a strong reduction in FCV protein synthesis. This effect could be reversed by the addition of full-length NCL but not by a δrNCL, lacking the N-terminal domain. Consistent with this, FCV infection of CrFK cells stably expressing δrNCL led to a reduction in virus protein translation. These results suggest that NCL is part of the FCV RNA translational complex, and that the N-terminal part of the protein is required for efficient FCV replication.
KW - AGRO100
KW - Aptamer
KW - FCV
KW - Nucleolin
KW - RNA virus
KW - Translation
KW - UTRs
UR - http://www.scopus.com/inward/record.url?scp=84949759363&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84949759363&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2015.12.001
DO - 10.1016/j.virol.2015.12.001
M3 - Article
C2 - 26707270
AN - SCOPUS:84949759363
SN - 0042-6822
VL - 489
SP - 51
EP - 62
JO - Virology
JF - Virology
ER -