Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe

W. Matthew Leevy; Seth T. Gammon; Hua Jiang; James R. Johnson; Dustin J. Maxwell; Erin N. Jackson; Manuel Marquez; David Piwnica-Worms; Bradley D. Smith

doi:10.1021/ja0665592

Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe

W. Matthew Leevy, Seth T. Gammon, Hua Jiang, James R. Johnson, Dustin J. Maxwell, Erin N. Jackson, Manuel Marquez, David Piwnica-Worms, Bradley D. Smith

Research output: Contribution to journal › Article › peer-review

234 Scopus citations

Abstract

An optical imaging probe was synthesized by attaching a near-infrared carbocyanine fluorophore to an affinity group containing two zinc(II) dipicolylamine (Zn-DPA) units. The probe has a strong and selective affinity for the surfaces of bacteria, and it was used to image infections of Gram-positive S. aureus and Gram-negative E. coli bacteria in living nude mice. After intravenous injection, the probe selectively accumulates at the sites of localized bacterial infections in the thigh muscles of the mice.

Original language	English (US)
Pages (from-to)	16476-16477
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	128
Issue number	51
DOIs	https://doi.org/10.1021/ja0665592
State	Published - Dec 27 2006
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja0665592

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title = "Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe",

abstract = "An optical imaging probe was synthesized by attaching a near-infrared carbocyanine fluorophore to an affinity group containing two zinc(II) dipicolylamine (Zn-DPA) units. The probe has a strong and selective affinity for the surfaces of bacteria, and it was used to image infections of Gram-positive S. aureus and Gram-negative E. coli bacteria in living nude mice. After intravenous injection, the probe selectively accumulates at the sites of localized bacterial infections in the thigh muscles of the mice.",

author = "Leevy, {W. Matthew} and Gammon, {Seth T.} and Hua Jiang and Johnson, {James R.} and Maxwell, {Dustin J.} and Jackson, {Erin N.} and Manuel Marquez and David Piwnica-Worms and Smith, {Bradley D.}",

year = "2006",

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T1 - Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe

AU - Leevy, W. Matthew

AU - Gammon, Seth T.

AU - Jiang, Hua

AU - Johnson, James R.

AU - Maxwell, Dustin J.

AU - Jackson, Erin N.

AU - Marquez, Manuel

AU - Piwnica-Worms, David

AU - Smith, Bradley D.

PY - 2006/12/27

Y1 - 2006/12/27

N2 - An optical imaging probe was synthesized by attaching a near-infrared carbocyanine fluorophore to an affinity group containing two zinc(II) dipicolylamine (Zn-DPA) units. The probe has a strong and selective affinity for the surfaces of bacteria, and it was used to image infections of Gram-positive S. aureus and Gram-negative E. coli bacteria in living nude mice. After intravenous injection, the probe selectively accumulates at the sites of localized bacterial infections in the thigh muscles of the mice.

AB - An optical imaging probe was synthesized by attaching a near-infrared carbocyanine fluorophore to an affinity group containing two zinc(II) dipicolylamine (Zn-DPA) units. The probe has a strong and selective affinity for the surfaces of bacteria, and it was used to image infections of Gram-positive S. aureus and Gram-negative E. coli bacteria in living nude mice. After intravenous injection, the probe selectively accumulates at the sites of localized bacterial infections in the thigh muscles of the mice.

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe

Abstract

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