TY - JOUR
T1 - Optimization of the liposomes encapsulating a new lipopeptide cgp 31362 for efficient activation of tumoricidal properties in monocytes and macrophages
AU - Nii, Akihiko
AU - Utsugi, Teruhiro
AU - Fan, Dominic
AU - Denkins, Yvonne
AU - Pak, Charles
AU - Brown, Dale
AU - van Hoogevest, Peter
AU - Fidler, Isaiah J.
PY - 1991/8
Y1 - 1991/8
N2 - The purpose of this study was to optimize a suitable liposomal carrier for CGP 31362, a new synthetic lipopeptide analogue of gram-negative bacterial cell walls. CGP 31362 was inserted into the membranes of different liposomes with different phospholipid composition. We determined the ability of these liposomes to activate tumoricidal properties in mouse peritoneal and bone marrow macrophages, and in human monocytes. The ideal liposome carrier for CGP 31362 consisted of phosphatidylcholine and phosphatidylserine in a 7:3 molar ratio. Subsequent to efficient binding and endocytosis, CGP 31362 in liposomes of this composition rendered mouse macrophages and human monocytes highly tumoricidal. Moreover, even in the absence of interferon-gamma, human monocytes released significant levels of tumor necrosis factor and interleukin-1. These data show that in a suitable liposomal carrier, the new synthetic lipopeptide in liposomes is a potent activator of tumoricidal properties in macrophages.
AB - The purpose of this study was to optimize a suitable liposomal carrier for CGP 31362, a new synthetic lipopeptide analogue of gram-negative bacterial cell walls. CGP 31362 was inserted into the membranes of different liposomes with different phospholipid composition. We determined the ability of these liposomes to activate tumoricidal properties in mouse peritoneal and bone marrow macrophages, and in human monocytes. The ideal liposome carrier for CGP 31362 consisted of phosphatidylcholine and phosphatidylserine in a 7:3 molar ratio. Subsequent to efficient binding and endocytosis, CGP 31362 in liposomes of this composition rendered mouse macrophages and human monocytes highly tumoricidal. Moreover, even in the absence of interferon-gamma, human monocytes released significant levels of tumor necrosis factor and interleukin-1. These data show that in a suitable liposomal carrier, the new synthetic lipopeptide in liposomes is a potent activator of tumoricidal properties in macrophages.
KW - Lipopeptide
KW - Liposomes
KW - Macrophage activation
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U2 - 10.1097/00002371-199108000-00002
DO - 10.1097/00002371-199108000-00002
M3 - Article
C2 - 1931862
AN - SCOPUS:0025915955
SN - 1524-9557
VL - 10
SP - 236
EP - 246
JO - Journal of Immunotherapy
JF - Journal of Immunotherapy
IS - 4
ER -