Optofluidic single molecule flow proteometry

Nan Jing, Chao Kai Chou, Mien Chie Hung, Jun Kameoka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A microfluidic single molecule fluorescence-based detection scheme is developed to identify target proteindirect from cell lysate by using polyclonal antibody. Relative concentration of target protein in solution is determined by twodimensional(2D) photon burst analysis. Compared to conventional ensemble measurement assays, this microfluidic single molecule approach combines the advantages of higher sensitivity, fast processing time, small sample consumption and high resolution quantitative analysis.

Original languageEnglish (US)
Title of host publicationSingle Molecule Spectroscopy and Imaging II
DOIs
StatePublished - 2009
EventSingle Molecule Spectroscopy and Imaging II - San Jose, CA, United States
Duration: Jan 24 2009Jan 25 2009

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7185
ISSN (Print)1605-7422

Other

OtherSingle Molecule Spectroscopy and Imaging II
Country/TerritoryUnited States
CitySan Jose, CA
Period1/24/091/25/09

Keywords

  • Flow proteometry
  • Fluorescence
  • Microfluidics
  • Single molecule detection

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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