Overexpression of interleukin 21 induces expansion of hematopoietic progenitor cells

Katsutoshi Ozaki; Ai Hishiya; Keiko Hatanaka; Hideaki Nakajima; Gang Wang; Patrick Hwu; Toshio Kitamura; Keiya Ozawa; Warren J. Leonard; Tetsuya Nosaka

doi:10.1532/IJH97.06036

Overexpression of interleukin 21 induces expansion of hematopoietic progenitor cells

Katsutoshi Ozaki, Ai Hishiya, Keiko Hatanaka, Hideaki Nakajima, Gang Wang, Patrick Hwu, Toshio Kitamura, Keiya Ozawa, Warren J. Leonard, Tetsuya Nosaka

Melanoma Medical Oncology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The interleukin 21 (IL-21) receptor is expressed on T-cells, B-cells, and natural killer cells, and IL-21 is critical for regulating immunoglobulin production in vivo in cooperation with IL-4. So far, however, little is known about a role for IL-21 outside the immune system. We investigated the effect of IL-21 on hematopoiesis in vivo by using the hydrodynamics gene-delivery method. Overexpression of IL-21 increases Sca-1⁺ cells in the periphery and spleen. It also increases the numbers of C-Kit⁺, Sca-1⁺, and lineage^-/low (KSL) cells and colony-forming units-granulocyte- macrophage (CFU-GM) in the spleen, indicating the expansion of hematopoietic progenitor cells. We found that even in RAG2^-/- mice, which lack mature T-cells and B-cells, IL-21 induced an increase in KSL cells and CFU-GM in the spleen. These results demonstrate that IL-21 can induce the expansion of hematopoietic progenitor cells in vivo, even in the absence of mature T-cells and B-cells.

Original language	English (US)
Pages (from-to)	224-230
Number of pages	7
Journal	International journal of hematology
Volume	84
Issue number	3
DOIs	https://doi.org/10.1532/IJH97.06036
State	Published - Oct 2006

Keywords

CFU-GM
Expansion
Hematopoietic progenitor cells
Interleukin 21
Overexpression

ASJC Scopus subject areas

Hematology

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10.1532/IJH97.06036

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@article{a1f58dab5ec14612b15a71fa1794ccda,

title = "Overexpression of interleukin 21 induces expansion of hematopoietic progenitor cells",

abstract = "The interleukin 21 (IL-21) receptor is expressed on T-cells, B-cells, and natural killer cells, and IL-21 is critical for regulating immunoglobulin production in vivo in cooperation with IL-4. So far, however, little is known about a role for IL-21 outside the immune system. We investigated the effect of IL-21 on hematopoiesis in vivo by using the hydrodynamics gene-delivery method. Overexpression of IL-21 increases Sca-1+ cells in the periphery and spleen. It also increases the numbers of C-Kit+, Sca-1+, and lineage-/low (KSL) cells and colony-forming units-granulocyte- macrophage (CFU-GM) in the spleen, indicating the expansion of hematopoietic progenitor cells. We found that even in RAG2-/- mice, which lack mature T-cells and B-cells, IL-21 induced an increase in KSL cells and CFU-GM in the spleen. These results demonstrate that IL-21 can induce the expansion of hematopoietic progenitor cells in vivo, even in the absence of mature T-cells and B-cells.",

keywords = "CFU-GM, Expansion, Hematopoietic progenitor cells, Interleukin 21, Overexpression",

author = "Katsutoshi Ozaki and Ai Hishiya and Keiko Hatanaka and Hideaki Nakajima and Gang Wang and Patrick Hwu and Toshio Kitamura and Keiya Ozawa and Leonard, {Warren J.} and Tetsuya Nosaka",

note = "Funding Information: This work was supported by a grant from the Chugai Pharmaceutical Company. The research was supported in part by the Intramural Research Program of the National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA. We thank Dr. Satoshi Takaki for providing RAG2–/– mice. Funding Information: aDivision of Hematopoietic Factors and bDepartment of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan; cDepartment of Melanoma Medical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, USA; dLaboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; eDivision of Hematology, Jichi Medical University, Tochigi, Japan",

year = "2006",

month = oct,

doi = "10.1532/IJH97.06036",

language = "English (US)",

volume = "84",

pages = "224--230",

journal = "International journal of hematology",

issn = "0925-5710",

publisher = "Springer Japan",

number = "3",

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T1 - Overexpression of interleukin 21 induces expansion of hematopoietic progenitor cells

AU - Ozaki, Katsutoshi

AU - Hishiya, Ai

AU - Hatanaka, Keiko

AU - Nakajima, Hideaki

AU - Wang, Gang

AU - Hwu, Patrick

AU - Kitamura, Toshio

AU - Ozawa, Keiya

AU - Leonard, Warren J.

AU - Nosaka, Tetsuya

N1 - Funding Information: This work was supported by a grant from the Chugai Pharmaceutical Company. The research was supported in part by the Intramural Research Program of the National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA. We thank Dr. Satoshi Takaki for providing RAG2–/– mice. Funding Information: aDivision of Hematopoietic Factors and bDepartment of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan; cDepartment of Melanoma Medical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, USA; dLaboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; eDivision of Hematology, Jichi Medical University, Tochigi, Japan

PY - 2006/10

Y1 - 2006/10

N2 - The interleukin 21 (IL-21) receptor is expressed on T-cells, B-cells, and natural killer cells, and IL-21 is critical for regulating immunoglobulin production in vivo in cooperation with IL-4. So far, however, little is known about a role for IL-21 outside the immune system. We investigated the effect of IL-21 on hematopoiesis in vivo by using the hydrodynamics gene-delivery method. Overexpression of IL-21 increases Sca-1+ cells in the periphery and spleen. It also increases the numbers of C-Kit+, Sca-1+, and lineage-/low (KSL) cells and colony-forming units-granulocyte- macrophage (CFU-GM) in the spleen, indicating the expansion of hematopoietic progenitor cells. We found that even in RAG2-/- mice, which lack mature T-cells and B-cells, IL-21 induced an increase in KSL cells and CFU-GM in the spleen. These results demonstrate that IL-21 can induce the expansion of hematopoietic progenitor cells in vivo, even in the absence of mature T-cells and B-cells.

AB - The interleukin 21 (IL-21) receptor is expressed on T-cells, B-cells, and natural killer cells, and IL-21 is critical for regulating immunoglobulin production in vivo in cooperation with IL-4. So far, however, little is known about a role for IL-21 outside the immune system. We investigated the effect of IL-21 on hematopoiesis in vivo by using the hydrodynamics gene-delivery method. Overexpression of IL-21 increases Sca-1+ cells in the periphery and spleen. It also increases the numbers of C-Kit+, Sca-1+, and lineage-/low (KSL) cells and colony-forming units-granulocyte- macrophage (CFU-GM) in the spleen, indicating the expansion of hematopoietic progenitor cells. We found that even in RAG2-/- mice, which lack mature T-cells and B-cells, IL-21 induced an increase in KSL cells and CFU-GM in the spleen. These results demonstrate that IL-21 can induce the expansion of hematopoietic progenitor cells in vivo, even in the absence of mature T-cells and B-cells.

KW - CFU-GM

KW - Expansion

KW - Hematopoietic progenitor cells

KW - Interleukin 21

KW - Overexpression

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SN - 0925-5710

VL - 84

SP - 224

EP - 230

JO - International journal of hematology

JF - International journal of hematology

IS - 3

ER -

Overexpression of interleukin 21 induces expansion of hematopoietic progenitor cells

Abstract

Keywords

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