TY - JOUR
T1 - p190-B, a Rho-GTPase-activating protein, is differentially expressed in terminal end buds and breast cancer
AU - Chakravarty, Geetika
AU - Roy, Deana
AU - Gonzales, Maria
AU - Gay, Jason
AU - Contreras, Alejandro
AU - Rosen, Jeffrey M.
PY - 2000/7
Y1 - 2000/7
N2 - Microdissection and differential display PCR were used to identify genes preferentially expressed in the highly proliferative terminal end buds (TEBs) in the mammary gland of 45-day-old virgin rats. One clone exhibited 87% homology to the human p190-B gene encoding a novel Rho-Gap. Using in situ hybridization, p190-B was detected in both the TEBs and the terminal ducts, with the highest expression observed in the outer layer of TEBs. During normal mammary gland development, p190-B mRNA expression was highest in the virgin mammary gland and decreased during late pregnancy and lactation. Interestingly, increased levels of p190-B mRNA relative to the normal mammary gland were seen in a subset of routine mammary tumors that appeared to be less well differentiated and potentially more aggressive. Transient transfection of a p190-B expression construct into MCF-10A human mammary epithelial cells resulted in disruption of the actin cytoskeleton, which suggests a role for p190-B in regulating the signaling pathways that influence cell migration and invasion. These results suggest that p190-B may be required for virgin mammary gland development, and its aberrant expression may occur in breast cancer.
AB - Microdissection and differential display PCR were used to identify genes preferentially expressed in the highly proliferative terminal end buds (TEBs) in the mammary gland of 45-day-old virgin rats. One clone exhibited 87% homology to the human p190-B gene encoding a novel Rho-Gap. Using in situ hybridization, p190-B was detected in both the TEBs and the terminal ducts, with the highest expression observed in the outer layer of TEBs. During normal mammary gland development, p190-B mRNA expression was highest in the virgin mammary gland and decreased during late pregnancy and lactation. Interestingly, increased levels of p190-B mRNA relative to the normal mammary gland were seen in a subset of routine mammary tumors that appeared to be less well differentiated and potentially more aggressive. Transient transfection of a p190-B expression construct into MCF-10A human mammary epithelial cells resulted in disruption of the actin cytoskeleton, which suggests a role for p190-B in regulating the signaling pathways that influence cell migration and invasion. These results suggest that p190-B may be required for virgin mammary gland development, and its aberrant expression may occur in breast cancer.
UR - http://www.scopus.com/inward/record.url?scp=0033862387&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033862387&partnerID=8YFLogxK
M3 - Article
C2 - 10939588
AN - SCOPUS:0033862387
SN - 1044-9523
VL - 11
SP - 343
EP - 354
JO - Cell Growth and Differentiation
JF - Cell Growth and Differentiation
IS - 7
ER -