Abstract
Correlation of p53 gene/protein alterations with incidence of oncogene amplification, a potential marker of prognosis, was evaluated in 26 fresh breast cancer samples. p53 gene was analyzed by SSCP and DNA sequencing while p53 protein status was investigated by immunohistochemistry (IH). Amplification of c-erbB2/neu, c-myc, N-ras, int-2, hst, PRAD-1 and EGFR genes was studied by slot blot and in situ hybridizations. p53 alterations were found in 31% cases by SSCP and 42% by IH; gene amplification was detected in 27% cases. p53 gene alterations correlated significantly with gene amplification (p = 0.006) and also with higher S-phase index (p = 0.026), aneuploidy (p = 0.026) and negative progesterone receptor status (p = 0.043).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 955-962 |
| Number of pages | 8 |
| Journal | International journal of oncology |
| Volume | 6 |
| Issue number | 5 |
| State | Published - 1995 |
Keywords
- Breast cancer
- Flow cytometry
- Oncogenes
- p53
ASJC Scopus subject areas
- Oncology
- Cancer Research