TY - JOUR
T1 - Pancreatic acinar cells in monolayer culture
T2 - Direct trophic effects of caerulein in vitro
AU - Logsdon, C. D.
AU - Williams, J. A.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1986
Y1 - 1986
N2 - Adult mouse pancreatic acinar cells were cultured as monolayers on collagen gels. Cells plated in medium containing 10% fetal bovine serum (FBS), epidermal growth factor (1 nM), carbachol (1 μM), insulin (1 μM), and corticosterone (10 nM) showed adaptive and growth responses. An adaptive phase occurred over the first 4-5 days, during which there was an ~50% decrease in the content of protein and DNA in the cultures, and the remaining attached cells showed reduced contents of zymogen granules. Subsequently, the cells spread out, divided, and formed confluent monolayers by days 11-14. Cell division was indicated by a doubling of the content of protein and DNA between days 5 and 9 and a 13-fold increase in thymidine incorporation. Microscopic examination of 14-day cultures revealed monolayers of cuboidal cells with morphological features of pancreatic acinar cells. To determine the direct effects of cholecystokinin on pancreatic acinar cell growth, cells were cultivated in media lacking added hormones and containing only 2.5% FBS with or without caerulein, a cholecystokinin analogue. Caerulein led to a 152% increase in DNA and an 89% increase in protein at day 9. By use of a preincubation [3H]thymidine incorporation assay, caerulein induced a dose-dependent increase in [3H]thymidine incorporation and nuclear labeling index, which was detectable at 0.1 nM, one-half maximal at 1 nM, and a maximal threefold increase occurred at 10 nM concentration. The results demonstrate a direct effect of caerulein on the pancreas to induce cell growth.
AB - Adult mouse pancreatic acinar cells were cultured as monolayers on collagen gels. Cells plated in medium containing 10% fetal bovine serum (FBS), epidermal growth factor (1 nM), carbachol (1 μM), insulin (1 μM), and corticosterone (10 nM) showed adaptive and growth responses. An adaptive phase occurred over the first 4-5 days, during which there was an ~50% decrease in the content of protein and DNA in the cultures, and the remaining attached cells showed reduced contents of zymogen granules. Subsequently, the cells spread out, divided, and formed confluent monolayers by days 11-14. Cell division was indicated by a doubling of the content of protein and DNA between days 5 and 9 and a 13-fold increase in thymidine incorporation. Microscopic examination of 14-day cultures revealed monolayers of cuboidal cells with morphological features of pancreatic acinar cells. To determine the direct effects of cholecystokinin on pancreatic acinar cell growth, cells were cultivated in media lacking added hormones and containing only 2.5% FBS with or without caerulein, a cholecystokinin analogue. Caerulein led to a 152% increase in DNA and an 89% increase in protein at day 9. By use of a preincubation [3H]thymidine incorporation assay, caerulein induced a dose-dependent increase in [3H]thymidine incorporation and nuclear labeling index, which was detectable at 0.1 nM, one-half maximal at 1 nM, and a maximal threefold increase occurred at 10 nM concentration. The results demonstrate a direct effect of caerulein on the pancreas to induce cell growth.
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U2 - 10.1152/ajpgi.1986.250.4.g440
DO - 10.1152/ajpgi.1986.250.4.g440
M3 - Article
C2 - 2421585
AN - SCOPUS:0022508059
SN - 0193-1857
VL - 250
SP - G440-G447
JO - American Journal of Physiology - Gastrointestinal and Liver Physiology
JF - American Journal of Physiology - Gastrointestinal and Liver Physiology
IS - 4 (13/4)
ER -