PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

F. Benavides; E. Glasscock; L. G. Coghlan; M. C. Stern; D. A. Weiss; C. J. Conti

doi:10.1258/0023677011911534

PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

F. Benavides, E. Glasscock, L. G. Coghlan, M. C. Stern, D. A. Weiss, C. J. Conti

Epigenetics & Molecular Carcinogenesis

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.

Original language	English (US)
Pages (from-to)	157-162
Number of pages	6
Journal	Laboratory Animals
Volume	35
Issue number	2
DOIs	https://doi.org/10.1258/0023677011911534
State	Published - Apr 1 2001

Keywords

Genetic monitoring
Inbred mice
Microsatellites
SENCAR mice

ASJC Scopus subject areas

Animal Science and Zoology
General Veterinary

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10.1258/0023677011911534

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abstract = "Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.",

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T1 - PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

AU - Benavides, F.

AU - Glasscock, E.

AU - Coghlan, L. G.

AU - Stern, M. C.

AU - Weiss, D. A.

AU - Conti, C. J.

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N2 - Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.

AB - Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.

KW - Genetic monitoring

KW - Inbred mice

KW - Microsatellites

KW - SENCAR mice

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PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

Abstract

Keywords

ASJC Scopus subject areas

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