TY - JOUR
T1 - Persistent shedding of viable SARS-CoV in urine and stool of SARS patients during the convalescent phase
AU - Xu, D.
AU - Zhang, Z.
AU - Jin, L.
AU - Chu, F.
AU - Mao, Y.
AU - Wang, H.
AU - Liu, M.
AU - Wang, M.
AU - Zhang, L.
AU - Gao, G. F.
AU - Wang, F. S.
N1 - Funding Information:
Acknowledgments We acknowledge the research funding from Beijing Natural Science Foundation (Number: 03F017) and Sino-UK Collaboration Foundation for SARS Immunopathogenesis Study (Number: H030230100130). We would like to thank Dr. P. Kretchmer for his assistance in editing this manuscript.
PY - 2005/3
Y1 - 2005/3
N2 - In order to further the present knowledge of the emerging severe acute respiratory syndrome-associated coronavirus (SARS-CoV), 486 different specimens from 54 patients with a clinical diagnosis of SARS were investigated for the presence of viral RNA, and 314 plasma specimens of 73 patients were examined for IgM and IgG antibodies specific against SARS-CoV using an indirect ELISA. Viral RNA was detectable in 28 of the 54 patients tested. Cumulative data showed that 67 of the 73 SARS patients demonstrated seroconversion by week 5 of illness. In contrast, only 1 of 278 healthy subjects enrolled in the study was found to be positive for the IgG antibody. Coexistence of viral RNA in plasma and specific antibodies was simultaneously observed over three consecutive weeks in two critical cases. In three convalescent patients in particular, cultivable SARS-CoV was detected in stool or urine specimens for longer than 4 weeks (29-36 days). These findings suggest that SARS-CoV may remain viable in the excretions of convalescent patients.
AB - In order to further the present knowledge of the emerging severe acute respiratory syndrome-associated coronavirus (SARS-CoV), 486 different specimens from 54 patients with a clinical diagnosis of SARS were investigated for the presence of viral RNA, and 314 plasma specimens of 73 patients were examined for IgM and IgG antibodies specific against SARS-CoV using an indirect ELISA. Viral RNA was detectable in 28 of the 54 patients tested. Cumulative data showed that 67 of the 73 SARS patients demonstrated seroconversion by week 5 of illness. In contrast, only 1 of 278 healthy subjects enrolled in the study was found to be positive for the IgG antibody. Coexistence of viral RNA in plasma and specific antibodies was simultaneously observed over three consecutive weeks in two critical cases. In three convalescent patients in particular, cultivable SARS-CoV was detected in stool or urine specimens for longer than 4 weeks (29-36 days). These findings suggest that SARS-CoV may remain viable in the excretions of convalescent patients.
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U2 - 10.1007/s10096-005-1299-5
DO - 10.1007/s10096-005-1299-5
M3 - Article
C2 - 15789222
AN - SCOPUS:20244387843
SN - 0934-9723
VL - 24
SP - 165
EP - 171
JO - European Journal of Clinical Microbiology and Infectious Diseases
JF - European Journal of Clinical Microbiology and Infectious Diseases
IS - 3
ER -