TY - JOUR
T1 - Perspectives on the DNA damage and replication checkpoint responses in Saccharomyces cerevisiae
AU - Putnam, Christopher D.
AU - Jaehnig, Eric J.
AU - Kolodner, Richard D.
N1 - Funding Information:
The authors thank Jorrit Ensernik, Huilin Zhou, and Wolf-Dietrich Heyer for their generous comments. Supported by NIH grants GM26017 and ES014811.
PY - 2009/9/2
Y1 - 2009/9/2
N2 - The DNA damage and replication checkpoints are believed to primarily slow the progression of the cell cycle to allow DNA repair to occur. Here we summarize known aspects of the Saccharomyces cerevisiae checkpoints including how these responses are integrated into downstream effects on the cell cycle, chromatin, DNA repair, and cytoplasmic targets. Analysis of the transcriptional response demonstrates that it is far more complex and less relevant to the repair of DNA damage than the bacterial SOS response. We also address more speculative questions regarding potential roles of the checkpoint during the normal S-phase and how current evidence hints at a checkpoint activation mechanism mediated by positive feedback that amplifies initial damage signals above a minimum threshold.
AB - The DNA damage and replication checkpoints are believed to primarily slow the progression of the cell cycle to allow DNA repair to occur. Here we summarize known aspects of the Saccharomyces cerevisiae checkpoints including how these responses are integrated into downstream effects on the cell cycle, chromatin, DNA repair, and cytoplasmic targets. Analysis of the transcriptional response demonstrates that it is far more complex and less relevant to the repair of DNA damage than the bacterial SOS response. We also address more speculative questions regarding potential roles of the checkpoint during the normal S-phase and how current evidence hints at a checkpoint activation mechanism mediated by positive feedback that amplifies initial damage signals above a minimum threshold.
KW - Cell cycle checkpoint
KW - DNA damage
KW - DNA repair
KW - Genome stability
KW - Positive feedback
KW - Transcriptional response
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U2 - 10.1016/j.dnarep.2009.04.021
DO - 10.1016/j.dnarep.2009.04.021
M3 - Article
C2 - 19477695
AN - SCOPUS:68249099217
SN - 1568-7864
VL - 8
SP - 974
EP - 982
JO - DNA Repair
JF - DNA Repair
IS - 9
ER -