TY - JOUR
T1 - Phase I study of intraventricular infusions of autologous ex vivo expanded NK cells in children with recurrent medulloblastoma and ependymoma
AU - Khatua, Soumen
AU - Cooper, Laurence J.N.
AU - Sandberg, David I.
AU - Ketonen, Leena
AU - Johnson, Jason M.
AU - Rytting, Michael E.
AU - Liu, Diane D.
AU - Meador, Heather
AU - Trikha, Prashant
AU - Nakkula, Robin J.
AU - Behbehani, Gregory K.
AU - Ragoonanan, Dristhi
AU - Gupta, Sumit
AU - Kotrotsou, Aikaterini
AU - Idris, Tagwa
AU - Shpall, Elizabeth J.
AU - Rezvani, Katy
AU - Colen, Rivka
AU - Zaky, Wafik
AU - Lee, Dean A.
AU - Gopalakrishnan, Vidya
N1 - Publisher Copyright:
© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Background. Recurrent pediatric medulloblastoma and ependymoma have a grim prognosis. We report a first-inhuman, phase I study of intraventricular infusions of ex vivo expanded autologous natural killer (NK) cells in these tumors, with correlative studies. Methods. Twelve patients were enrolled, 9 received protocol therapy up to 3 infusions weekly, in escalating doses from 3 × 106 to 3 × 108 NK cells/m2/infusion, for up to 3 cycles. Cerebrospinal fluid (CSF) was obtained for cellular profile, persistence, and phenotypic analysis of NK cells. Radiomic characterization on pretreatment MRI scans was performed in 7 patients, to develop a non-invasive imaging-based signature. Results. Primary objectives of NK cell harvest, expansion, release, and safety of 112 intraventricular infusions of NK cells were achieved in all 9 patients. There were no dose-limiting toxicities. All patients showed progressive disease (PD), except 1 patient showed stable disease for one month at end of study follow-up. Another patient had transient radiographic response of the intraventricular tumor after 5 infusions of NK cell before progressing to PD. At higher dose levels, NK cells increased in the CSF during treatment with repetitive infusions (mean 11.6-fold). Frequent infusions of NK cells resulted in CSF pleocytosis. Radiomic signatures were profiled in 7 patients, evaluating ability to predict upfront radiographic changes, although they did not attain statistical significance. Conclusions. This study demonstrated feasibility of production and safety of intraventricular infusions of autologous NK cells. These findings support further investigation of locoregional NK cell infusions in children with brain malignancies.
AB - Background. Recurrent pediatric medulloblastoma and ependymoma have a grim prognosis. We report a first-inhuman, phase I study of intraventricular infusions of ex vivo expanded autologous natural killer (NK) cells in these tumors, with correlative studies. Methods. Twelve patients were enrolled, 9 received protocol therapy up to 3 infusions weekly, in escalating doses from 3 × 106 to 3 × 108 NK cells/m2/infusion, for up to 3 cycles. Cerebrospinal fluid (CSF) was obtained for cellular profile, persistence, and phenotypic analysis of NK cells. Radiomic characterization on pretreatment MRI scans was performed in 7 patients, to develop a non-invasive imaging-based signature. Results. Primary objectives of NK cell harvest, expansion, release, and safety of 112 intraventricular infusions of NK cells were achieved in all 9 patients. There were no dose-limiting toxicities. All patients showed progressive disease (PD), except 1 patient showed stable disease for one month at end of study follow-up. Another patient had transient radiographic response of the intraventricular tumor after 5 infusions of NK cell before progressing to PD. At higher dose levels, NK cells increased in the CSF during treatment with repetitive infusions (mean 11.6-fold). Frequent infusions of NK cells resulted in CSF pleocytosis. Radiomic signatures were profiled in 7 patients, evaluating ability to predict upfront radiographic changes, although they did not attain statistical significance. Conclusions. This study demonstrated feasibility of production and safety of intraventricular infusions of autologous NK cells. These findings support further investigation of locoregional NK cell infusions in children with brain malignancies.
KW - Immunotherapy
KW - Intraventricular infusions
KW - Natural killer cells
KW - Recurrent brain tumors
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U2 - 10.1093/neuonc/noaa047
DO - 10.1093/neuonc/noaa047
M3 - Article
C2 - 32152626
AN - SCOPUS:85089555702
SN - 1522-8517
VL - 22
SP - 1214
EP - 1225
JO - Neuro-oncology
JF - Neuro-oncology
IS - 8
ER -