TY - JOUR
T1 - Phase I/II study of G3139 (Bcl-2 antisense oligonucleotide) in combination with doxorubicin and docetaxel in breast cancer
AU - Moulder, Stacy L.
AU - Symmans, W. Fraser
AU - Booser, Daniel J.
AU - Madden, Timothy L.
AU - Lipsanen, Cindy
AU - Yuan, Linda
AU - Brewster, Abenaa M.
AU - Cristofanilli, Massimo
AU - Hunt, Kelly K.
AU - Buchholz, Thomas A.
AU - Zwiebel, James
AU - Valero, Vicente
AU - Hortobagyi, Gabriel N.
AU - Esteva, Francisco J.
PY - 2008/12/1
Y1 - 2008/12/1
N2 - Purpose:Preclinical data showed enhancement of breast cancer cell death when G3139 was combined with anthracyclines and taxanes. We evaluated the efficacy and safety of a Bcl-2 antisense oligonucleotide, G3139, in combination with doxorubicin (A) and docetaxel (T) in patients with locally advanced breast cancer (LABC). Experimental Design:Following a brief phase I to determine the phase II dose, patients with locally advanced breast cancer received G3139 administered by continuous i.v. infusion for 5 to 7days with bolus A(50 mg/m 2) andT (75 mg/m 2) administered on either day 3 or 6 of therapy with G3139. Cycles were repeated every 21 days ×6 in the neoadjuvant setting. Serial plasma samples were obtained for pharmacokinetic analysis. Tissue samples were obtained before and after therapy for pharmacodynamic analysis of Bcl-2 expression. Results:Thirty patients (median age, 49 years;range, 24-71 years) received 160 cycles. During the phase I portion of the trial, the dose of G3139 was escalated from 3 to 7 mg/kg/d (i.v. for 5 days) in combination with AT During the phase II portion of the trial, several doses and schedules of G3139 were evaluated. There were no pathologic complete responses. Pharmacodynamic studies showed limited Bcl-2 down-regulation in the primary tumors. Conclusions:G3139 in combination with doxorubicin and docetaxel is well tolerated. No pathologic complete response was seen and pharmacodynamic studies showed very little down-regulation of Bcl-2 in primary tumors, perhaps related to issues with insufficient drug delivery to the intact tumor.
AB - Purpose:Preclinical data showed enhancement of breast cancer cell death when G3139 was combined with anthracyclines and taxanes. We evaluated the efficacy and safety of a Bcl-2 antisense oligonucleotide, G3139, in combination with doxorubicin (A) and docetaxel (T) in patients with locally advanced breast cancer (LABC). Experimental Design:Following a brief phase I to determine the phase II dose, patients with locally advanced breast cancer received G3139 administered by continuous i.v. infusion for 5 to 7days with bolus A(50 mg/m 2) andT (75 mg/m 2) administered on either day 3 or 6 of therapy with G3139. Cycles were repeated every 21 days ×6 in the neoadjuvant setting. Serial plasma samples were obtained for pharmacokinetic analysis. Tissue samples were obtained before and after therapy for pharmacodynamic analysis of Bcl-2 expression. Results:Thirty patients (median age, 49 years;range, 24-71 years) received 160 cycles. During the phase I portion of the trial, the dose of G3139 was escalated from 3 to 7 mg/kg/d (i.v. for 5 days) in combination with AT During the phase II portion of the trial, several doses and schedules of G3139 were evaluated. There were no pathologic complete responses. Pharmacodynamic studies showed limited Bcl-2 down-regulation in the primary tumors. Conclusions:G3139 in combination with doxorubicin and docetaxel is well tolerated. No pathologic complete response was seen and pharmacodynamic studies showed very little down-regulation of Bcl-2 in primary tumors, perhaps related to issues with insufficient drug delivery to the intact tumor.
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U2 - 10.1158/1078-0432.CCR-08-1104
DO - 10.1158/1078-0432.CCR-08-1104
M3 - Article
C2 - 19047121
AN - SCOPUS:59449091820
SN - 1078-0432
VL - 14
SP - 7909
EP - 7916
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 23
ER -