TY - JOUR
T1 - Phenotype and function of a CD56+ peripheral blood monocyte
AU - Sconocchia, G.
AU - Keyvanfar, K.
AU - El Ouriaghli, F.
AU - Grube, M.
AU - Rezvani, K.
AU - Fujiwara, H.
AU - McCoy, J. P.
AU - Hensel, N.
AU - Barrett, A. J.
PY - 2005/1
Y1 - 2005/1
N2 - G-CSF primed CD34 cells cultured for 2-3 weeks in IL-2 and stem cell factor generate CD56high cells with phenotypic and morphologic features of NK cells, and a novel adherent CD56low CD16- population expressing myeloid markers (CD33 and HLA-DR). We hypothesized that similar cells might also occur in peripheral blood. In 13/13 normal individuals, we found a circulating population of CD56low, CD33+, FcγRI+, FcγRII+, HLA-DR+, CD11bhigh, CD14+ monocytes closely resembling the cultured CD56lowCD33+ cells. They may represent a normal counterpart of the CD56+ CD33+ hybrid myeloid/natural killer cell leukemia. Their mean frequency was 1.3±1% (standard deviation), range 0.16-3.5%, of total mononuclear cells. CD56lowCD33+ cells, primed with cytomegalovirus antigen, induced autologous T-lymphocyte proliferation comparably to CD56-, CD14+ peripheral blood monocytes (PBM). Conversely, CD56low cells induced greater T-cell proliferation than CD56- PBM when lymphocyte responders were HLA mismatched. Unstimulated CD56lowCD33+ cells showed a low antiproliferative effect on K562, which was increased upon LPS stimulation. The pattern of cytokine production by CD56lowCD33+ cells and PBM largely overlapped; however, they produced detectable levels of IL-6 and IL-1β. These results define a minor monocyte population with distinct phenotypic and functional features.
AB - G-CSF primed CD34 cells cultured for 2-3 weeks in IL-2 and stem cell factor generate CD56high cells with phenotypic and morphologic features of NK cells, and a novel adherent CD56low CD16- population expressing myeloid markers (CD33 and HLA-DR). We hypothesized that similar cells might also occur in peripheral blood. In 13/13 normal individuals, we found a circulating population of CD56low, CD33+, FcγRI+, FcγRII+, HLA-DR+, CD11bhigh, CD14+ monocytes closely resembling the cultured CD56lowCD33+ cells. They may represent a normal counterpart of the CD56+ CD33+ hybrid myeloid/natural killer cell leukemia. Their mean frequency was 1.3±1% (standard deviation), range 0.16-3.5%, of total mononuclear cells. CD56lowCD33+ cells, primed with cytomegalovirus antigen, induced autologous T-lymphocyte proliferation comparably to CD56-, CD14+ peripheral blood monocytes (PBM). Conversely, CD56low cells induced greater T-cell proliferation than CD56- PBM when lymphocyte responders were HLA mismatched. Unstimulated CD56lowCD33+ cells showed a low antiproliferative effect on K562, which was increased upon LPS stimulation. The pattern of cytokine production by CD56lowCD33+ cells and PBM largely overlapped; however, they produced detectable levels of IL-6 and IL-1β. These results define a minor monocyte population with distinct phenotypic and functional features.
KW - Cellular differentiation
KW - Cellular proliferation
KW - Macrophages
KW - Monocytes
KW - NK cells
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U2 - 10.1038/sj.leu.2403550
DO - 10.1038/sj.leu.2403550
M3 - Article
C2 - 15526027
AN - SCOPUS:11844295984
SN - 0887-6924
VL - 19
SP - 69
EP - 76
JO - Leukemia
JF - Leukemia
IS - 1
ER -