TY - JOUR
T1 - Phenotypic Analysis of Periimplantation to Mid-Gestation Lethality in Mice
AU - Papaioannou, Virginia E.
AU - Behringer, Richard R.
N1 - Publisher Copyright:
© 2023 Cold Spring Harbor Laboratory Press.
PY - 2023/12
Y1 - 2023/12
N2 - Periimplantation to mid-gestation lethality is indicated if no living homozygous mutants are recovered at E12.5 and the number of empty implantation sites or degenerating/abnormal embryos fits the expected number of homozygous mutants. To determine the time of death, this overview details the characteristic features of lethality shortly after implantation (E4.5-E5.5) or lethality between gastrulation and allantoic fusion (E6.5-E9.5). Determining the phenotype of the mutants involves making a gross morphological assessment, staging the embryos, and photodocumenting any abnormalities. Further levels of analysis discussed are histological assessment, molecular characterization of gene expression in the mutant embryos, and measurements of cell proliferation and cell death.
AB - Periimplantation to mid-gestation lethality is indicated if no living homozygous mutants are recovered at E12.5 and the number of empty implantation sites or degenerating/abnormal embryos fits the expected number of homozygous mutants. To determine the time of death, this overview details the characteristic features of lethality shortly after implantation (E4.5-E5.5) or lethality between gastrulation and allantoic fusion (E6.5-E9.5). Determining the phenotype of the mutants involves making a gross morphological assessment, staging the embryos, and photodocumenting any abnormalities. Further levels of analysis discussed are histological assessment, molecular characterization of gene expression in the mutant embryos, and measurements of cell proliferation and cell death.
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U2 - 10.1101/pdb.over107972
DO - 10.1101/pdb.over107972
M3 - Review article
C2 - 37932076
AN - SCOPUS:85181986150
SN - 1940-3402
VL - 2023
SP - 951
EP - 965
JO - Cold Spring Harbor protocols
JF - Cold Spring Harbor protocols
IS - 12
ER -