Phosphorylation of v-mos Ser 47 by the mitotic form of p34(cdc2)

W. Bai, Balraj Singh, W. L. Karshin, R. A. Shonk, R. B. Arlinghaus

Research output: Contribution to journalArticle

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Abstract

P85(gag-mos) is hyperphosphorylated during mitosis in normal rat kidney (NRK) cells transformed by Moloney murine sarcoma virus ts110. We now report that P85(gag-mos) is phosphorylated in vitro by the mitotic form of the cdc2 kinase (p34(cdc2) known as M-phase kinase) derived from virus-transformed cells. The major site of P85(gag-mos) phosphorylation by the M-phase kinase in vitro lies within the amino-terminal portion of the viral mos protein sequence spanning residues 45-53, as determined by tryptic peptide mapping. A synthetic peptide corresponding to amino acids 37-55 of v-mos was specifically phosphorylated by the M-phase kinase, whereas v-mos peptides either lacking Ser 47 or substituted with Ala at residue 47 were not phosphorylated. Protein sequencing analyses established that the M-phase kinase specifically phosphorylates Ser 47. Tryptic phosphopeptide mapping of the in vivo-phosphorylated gag-mos protein from mitotic cells indicated that the 45-53 v-mos region was also phosphorylated within mitotic cells. These findings demonstrate that the M-phase kinase phosphorylates the viral mos protein at Ser 47. These results were unexpected in view of earlier reports regarding cdc2 kinase activation/stabilization by the c-mos kinase in maturing oocytes.

Original languageEnglish (US)
Pages (from-to)1715-1723
Number of pages9
JournalOncogene
Volume6
Issue number10
StatePublished - 1991

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Phosphotransferases
Phosphorylation
Oncogene Proteins v-mos
Cell Division
Viral Proteins
Moloney murine sarcoma virus
gag Gene Products
Phosphopeptides
Peptides
Peptide Mapping
Protein Sequence Analysis
Mitosis
Oocytes
Viruses
Kidney
Amino Acids

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Bai, W., Singh, B., Karshin, W. L., Shonk, R. A., & Arlinghaus, R. B. (1991). Phosphorylation of v-mos Ser 47 by the mitotic form of p34(cdc2). Oncogene, 6(10), 1715-1723.

Phosphorylation of v-mos Ser 47 by the mitotic form of p34(cdc2). / Bai, W.; Singh, Balraj; Karshin, W. L.; Shonk, R. A.; Arlinghaus, R. B.

In: Oncogene, Vol. 6, No. 10, 1991, p. 1715-1723.

Research output: Contribution to journalArticle

Bai, W, Singh, B, Karshin, WL, Shonk, RA & Arlinghaus, RB 1991, 'Phosphorylation of v-mos Ser 47 by the mitotic form of p34(cdc2)', Oncogene, vol. 6, no. 10, pp. 1715-1723.
Bai W, Singh B, Karshin WL, Shonk RA, Arlinghaus RB. Phosphorylation of v-mos Ser 47 by the mitotic form of p34(cdc2). Oncogene. 1991;6(10):1715-1723.
Bai, W. ; Singh, Balraj ; Karshin, W. L. ; Shonk, R. A. ; Arlinghaus, R. B. / Phosphorylation of v-mos Ser 47 by the mitotic form of p34(cdc2). In: Oncogene. 1991 ; Vol. 6, No. 10. pp. 1715-1723.
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