TY - JOUR
T1 - Polarographic pO2 measurement in mice
T2 - Effect of tumor type, site of implantation, and anesthesia
AU - Milross, Christopher G.
AU - Peters, Lester J.
AU - Hunter, Nancy R.
AU - Mason, Kathryn A.
AU - Tucker, Susan L.
AU - Milas, Luka
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 1996
Y1 - 1996
N2 - In this study we investigated how polarographically measured pO2 profiles of experimental tumors were affected by histological type, site of tumor implantation, and the use of two different anesthetic agents, sodium pentobarbital and ketamine with xylazine. Oxygen measurements were obtained in four syngeneic murine tumors, the sarcomas Sa-NH and FSa-II, the mammary carcinoma MCa-4, and the ovarian carcinoma OCa-I, and in normal muscle. Comparison of the pO2 measurements revealed significant differences between various groups in both the percentage of pO2 measurements less than or equal to 2.5 mm Hg, and the median pO2 OCa-I was significantly more hypoxic than the other tumors. Three of the four tumors growing subcutaneously were numerically less well oxygenated than their intramuscular counterparts, significantly so in the case of MCa-4. Both types of anesthesia significantly reduced measured pO2 for intramuscular Sa-NH and MCa-4. It is essential that variables such as tumor type, tumor site, and anesthesia be controlled for when interpreting the radiobiological significance of pO2 measurements and when comparing results obtained in different laboratories. Furthermore, these observations imply the need for strict standardization of clinical protocols in order for pO2 measurements to have any real prospect as a predictive assay.
AB - In this study we investigated how polarographically measured pO2 profiles of experimental tumors were affected by histological type, site of tumor implantation, and the use of two different anesthetic agents, sodium pentobarbital and ketamine with xylazine. Oxygen measurements were obtained in four syngeneic murine tumors, the sarcomas Sa-NH and FSa-II, the mammary carcinoma MCa-4, and the ovarian carcinoma OCa-I, and in normal muscle. Comparison of the pO2 measurements revealed significant differences between various groups in both the percentage of pO2 measurements less than or equal to 2.5 mm Hg, and the median pO2 OCa-I was significantly more hypoxic than the other tumors. Three of the four tumors growing subcutaneously were numerically less well oxygenated than their intramuscular counterparts, significantly so in the case of MCa-4. Both types of anesthesia significantly reduced measured pO2 for intramuscular Sa-NH and MCa-4. It is essential that variables such as tumor type, tumor site, and anesthesia be controlled for when interpreting the radiobiological significance of pO2 measurements and when comparing results obtained in different laboratories. Furthermore, these observations imply the need for strict standardization of clinical protocols in order for pO2 measurements to have any real prospect as a predictive assay.
KW - anesthesia
KW - histograph
KW - pO
KW - polarography
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U2 - 10.1002/(SICI)1520-6823(1996)4:3<108::AID-ROI2>3.0.CO;2-1
DO - 10.1002/(SICI)1520-6823(1996)4:3<108::AID-ROI2>3.0.CO;2-1
M3 - Article
AN - SCOPUS:0029744838
SN - 1065-7541
VL - 4
SP - 108
EP - 114
JO - Radiation Oncology Investigations
JF - Radiation Oncology Investigations
IS - 3
ER -