TY - JOUR
T1 - Polyamine measurements in the uterine cervix
AU - Mitchell, Michele Follen
AU - Tortolero-Luna, Guillermo
AU - Lee, J. Jack
AU - Hittelman, Walter K.
AU - Lotan, Reuben
AU - Wharton, J. Taylor
AU - Hong, Waun K.
AU - Nishioka, Kenji
PY - 1997
Y1 - 1997
N2 - Cervical cancer remains a significant health problem. New strategies based on the molecular aspects of cervical carcinogenesis are needed. Chemoprevention represents a novel strategy for cervical cancer prevention. Our group plans phase I and II trials using α-difluoromethylornithine, a suicide inhibitor of ornithine decarboxylase and potent antiproliferative chemopreventive agent. We conducted a study to identify which polyamines in tissue could best serve as surrogate endpoint biomarkers for future trials. Thirty patients with biopsy-proven cervical intraepithelial neoplasia grade 3 underwent colposcopically directed biopsies of normal and abnormal areas of the uterine cervix for analysis of polyamine synthesis biomarkers. Statistically significant differences were found in the ornithine decarboxylase value and the spermidine:spermine ratio between normal and abnormal areas of the cervix. In general, the ranges in measurements varied widely. Differences in polyamine synthesis biomarkers between colposcopically normal and abnormal areas can be demonstrated. However, studies using polyamine synthesis biomarkers in the cervix would require large numbers of patients to achieve significance.
AB - Cervical cancer remains a significant health problem. New strategies based on the molecular aspects of cervical carcinogenesis are needed. Chemoprevention represents a novel strategy for cervical cancer prevention. Our group plans phase I and II trials using α-difluoromethylornithine, a suicide inhibitor of ornithine decarboxylase and potent antiproliferative chemopreventive agent. We conducted a study to identify which polyamines in tissue could best serve as surrogate endpoint biomarkers for future trials. Thirty patients with biopsy-proven cervical intraepithelial neoplasia grade 3 underwent colposcopically directed biopsies of normal and abnormal areas of the uterine cervix for analysis of polyamine synthesis biomarkers. Statistically significant differences were found in the ornithine decarboxylase value and the spermidine:spermine ratio between normal and abnormal areas of the cervix. In general, the ranges in measurements varied widely. Differences in polyamine synthesis biomarkers between colposcopically normal and abnormal areas can be demonstrated. However, studies using polyamine synthesis biomarkers in the cervix would require large numbers of patients to achieve significance.
KW - Cervical intraepithelial neoplasia (CIN)
KW - Chemoprevention
KW - Surrogate endpoint biomarker (SEB)
KW - α-difluoromethylornithine (DFMO)
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U2 - 10.1002/(SICI)1097-4644(1997)28/29+<125::AID-JCB14>3.0.CO;2-G
DO - 10.1002/(SICI)1097-4644(1997)28/29+<125::AID-JCB14>3.0.CO;2-G
M3 - Article
C2 - 9589357
AN - SCOPUS:0031401653
SN - 0730-2312
VL - 67
SP - 125
EP - 132
JO - Journal of cellular biochemistry
JF - Journal of cellular biochemistry
IS - SUPPL. 28/29
ER -