Polymorphism at codon 72 of the p53 gene in human acute myelogenous leukemia

Wei Zhang; Guiying Hu; Albert Deisseroth

doi:10.1016/0378-1119(92)90738-B

Polymorphism at codon 72 of the p53 gene in human acute myelogenous leukemia

Wei Zhang, Guiying Hu, Albert Deisseroth

Pathology

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

A common polymorphism at codon 72 of the p53 gene in patients with acute myelogenous leukemia (AML) was analyzed by single-strand conformation polymorphism assay and sodium dodecyl sulfate polyacrylamide-gel electrophoresis of immunoprecipitated ³⁵S-labeled P53 protein. No association between this polymorphism and a marked predisposition to AML was found. The half-lives of these two polymorphic forms of P53 were equivalent in normal phytohemagglutinin-stimulated lymphocytes, while the P53 Pro⁷² isoform was found to be twice as stable as the Arg⁷² isoform in Daudi cells.

Original language	English (US)
Pages (from-to)	271-275
Number of pages	5
Journal	Gene
Volume	117
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(92)90738-B
State	Published - Aug 15 1992

Keywords

Lymphocytes
allelic frequency
genetic predisposition
immunoprecipitation
isoform
stability

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(92)90738-B

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title = "Polymorphism at codon 72 of the p53 gene in human acute myelogenous leukemia",

abstract = "A common polymorphism at codon 72 of the p53 gene in patients with acute myelogenous leukemia (AML) was analyzed by single-strand conformation polymorphism assay and sodium dodecyl sulfate polyacrylamide-gel electrophoresis of immunoprecipitated 35S-labeled P53 protein. No association between this polymorphism and a marked predisposition to AML was found. The half-lives of these two polymorphic forms of P53 were equivalent in normal phytohemagglutinin-stimulated lymphocytes, while the P53 Pro72 isoform was found to be twice as stable as the Arg72 isoform in Daudi cells.",

keywords = "Lymphocytes, allelic frequency, genetic predisposition, immunoprecipitation, isoform, stability",

author = "Wei Zhang and Guiying Hu and Albert Deisseroth",

note = "Funding Information: We thank Drs. William Benedict and Hong-Ji Xu for their helpful comments. This work was supported by grants to Dr. Albert Deisseroth from the National Cancer Institute (PO1 CA49639-01A 1), the American Cancer Society (IM-580), the Sid Richardson Foundation, the Kleberg Foundation, the Ladies Leukemia League of Louisiana, the Bush Fund for Leukemia Research, and the Anderson Chair for Cancer Treatment and Research. Special thanks to Gerrie Zhang and Rosemarie Lauzon for their expert editorial assistance.",

year = "1992",

month = aug,

day = "15",

doi = "10.1016/0378-1119(92)90738-B",

language = "English (US)",

volume = "117",

pages = "271--275",

journal = "Gene",

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number = "2",

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TY - JOUR

T1 - Polymorphism at codon 72 of the p53 gene in human acute myelogenous leukemia

AU - Zhang, Wei

AU - Hu, Guiying

AU - Deisseroth, Albert

N1 - Funding Information: We thank Drs. William Benedict and Hong-Ji Xu for their helpful comments. This work was supported by grants to Dr. Albert Deisseroth from the National Cancer Institute (PO1 CA49639-01A 1), the American Cancer Society (IM-580), the Sid Richardson Foundation, the Kleberg Foundation, the Ladies Leukemia League of Louisiana, the Bush Fund for Leukemia Research, and the Anderson Chair for Cancer Treatment and Research. Special thanks to Gerrie Zhang and Rosemarie Lauzon for their expert editorial assistance.

PY - 1992/8/15

Y1 - 1992/8/15

N2 - A common polymorphism at codon 72 of the p53 gene in patients with acute myelogenous leukemia (AML) was analyzed by single-strand conformation polymorphism assay and sodium dodecyl sulfate polyacrylamide-gel electrophoresis of immunoprecipitated 35S-labeled P53 protein. No association between this polymorphism and a marked predisposition to AML was found. The half-lives of these two polymorphic forms of P53 were equivalent in normal phytohemagglutinin-stimulated lymphocytes, while the P53 Pro72 isoform was found to be twice as stable as the Arg72 isoform in Daudi cells.

AB - A common polymorphism at codon 72 of the p53 gene in patients with acute myelogenous leukemia (AML) was analyzed by single-strand conformation polymorphism assay and sodium dodecyl sulfate polyacrylamide-gel electrophoresis of immunoprecipitated 35S-labeled P53 protein. No association between this polymorphism and a marked predisposition to AML was found. The half-lives of these two polymorphic forms of P53 were equivalent in normal phytohemagglutinin-stimulated lymphocytes, while the P53 Pro72 isoform was found to be twice as stable as the Arg72 isoform in Daudi cells.

KW - Lymphocytes

KW - allelic frequency

KW - genetic predisposition

KW - immunoprecipitation

KW - isoform

KW - stability

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DO - 10.1016/0378-1119(92)90738-B

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AN - SCOPUS:0026649902

SN - 0378-1119

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JO - Gene

JF - Gene

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Polymorphism at codon 72 of the p53 gene in human acute myelogenous leukemia

Abstract

Keywords

ASJC Scopus subject areas

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