TY - JOUR
T1 - Polymorphism of DNA ligase I and risk of lung cancer - A case-control analysis
AU - Shen, Hongbing
AU - Spitz, Margaret R.
AU - Qiao, Yawei
AU - Zheng, Yuxin
AU - Hong, Waun K.
AU - Wei, Qingyi
N1 - Funding Information:
The authors would like to thank Dr Maureen Goode (Department of Scientific Publications) for editing the manuscript; Susan Honn for assistance in recruiting study subjects; Wayne Gosbee for assistance with data management and Joanne Sider and Joyce Brown for assistance in preparing the manuscript. This investigation was supported by NIH grants CA 55769 and CA 86390 (to M.R.S), CA 68437 (to W.K.H.) and ES 11740, CA 70334 and CA 74851 (to Q.W.) and by funds collected pursuant to the Comprehensive Tobacco Settlement of 1998 and appropriated by the 76th Legislature to The University of Texas M.D. Anderson Cancer Center.
PY - 2002
Y1 - 2002
N2 - DNA ligases catalyze the joining of single and double-strand DNA breaks, which is an essential step in DNA replication, recombination and repair. Recently, a common single nucleotide polymorphism (A→C) in exon 6 of DNA ligase I (LIG1) was identified, but its functional relevance remains to be determined. Because LIG1 participates in DNA repair and reduced DNA repair capacity is associated with risk of lung cancer, we evaluated in a non-population-based case-control study of 530 lung cancer cases and 570 cancer-free controls the role of this polymorphism in susceptibility to lung cancer. All of the subjects were non-Hispanic whites and the controls were frequency-matched to cases on age, sex and smoking status. Using the polymerase chain reaction-restriction fragment length polymorphism method, we found that this LIGI A→C substitution was very common in healthy controls and that the A and C allele frequencies were close to 0.5. However, there was no significant difference in the frequency distributions of LIGI genotypes between lung cancer cases and controls (25.7, 49.8 and 24.5% in cases and 26.1, 49.7 and 24.2% in controls for the AA, AC and CC genotypes, respectively). Therefore, there was no evidence to support an association between this polymorphism and the risk of lung cancer (adjusted odds ratio (OR)=1.06, 95% confidence interval (CI)=0.76-1.49 for AC versus CC and OR=0.93, 95% CI=0.64-1.36 for AA versus CC) neither in all cases nor in different histopathologic types. The results of this large case-control study suggest that this LIG1 polymorphism may not play an important role in susceptibility to lung cancer.
AB - DNA ligases catalyze the joining of single and double-strand DNA breaks, which is an essential step in DNA replication, recombination and repair. Recently, a common single nucleotide polymorphism (A→C) in exon 6 of DNA ligase I (LIG1) was identified, but its functional relevance remains to be determined. Because LIG1 participates in DNA repair and reduced DNA repair capacity is associated with risk of lung cancer, we evaluated in a non-population-based case-control study of 530 lung cancer cases and 570 cancer-free controls the role of this polymorphism in susceptibility to lung cancer. All of the subjects were non-Hispanic whites and the controls were frequency-matched to cases on age, sex and smoking status. Using the polymerase chain reaction-restriction fragment length polymorphism method, we found that this LIGI A→C substitution was very common in healthy controls and that the A and C allele frequencies were close to 0.5. However, there was no significant difference in the frequency distributions of LIGI genotypes between lung cancer cases and controls (25.7, 49.8 and 24.5% in cases and 26.1, 49.7 and 24.2% in controls for the AA, AC and CC genotypes, respectively). Therefore, there was no evidence to support an association between this polymorphism and the risk of lung cancer (adjusted odds ratio (OR)=1.06, 95% confidence interval (CI)=0.76-1.49 for AC versus CC and OR=0.93, 95% CI=0.64-1.36 for AA versus CC) neither in all cases nor in different histopathologic types. The results of this large case-control study suggest that this LIG1 polymorphism may not play an important role in susceptibility to lung cancer.
KW - DNA repair
KW - Genetic polymorphism
KW - Lung cancer
KW - Molecular epidemiology
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U2 - 10.1016/S0169-5002(01)00485-8
DO - 10.1016/S0169-5002(01)00485-8
M3 - Article
C2 - 12009232
AN - SCOPUS:0036257405
SN - 0169-5002
VL - 36
SP - 243
EP - 247
JO - Lung Cancer
JF - Lung Cancer
IS - 3
ER -